ArticlePlant biotechnology journal2023
Directed evolution rice genes with randomly multiplexed sgRNAs assembly of base editors.
Article in Plant biotechnology journal, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it, 27 citations in OpenAlex.
- Pooled it
- Enhancing CRISPR-Cas12a base editing in plants with LbCas12a variants and introns.Journal of integrative plant biology · 2026Article
- Optimized TadA-derived base editors efficiently manipulate mRNA splicing by A-to-G and C-to-K editing in potato.Journal of integrative plant biology · 2026Article
- Development of elite germplasm with high ɣ-aminobutyric acid (GABA) content by targeted mutation ofaBIOTECH · 2026Article
- CRISPR/Cas9-Mediated Base Editing of SiGS1 Confers Glufosinate Resistance in Foxtail Millet (Setaria italica).Plant biotechnology journal · 2026Article
- Cas9-Embedding Hyperactive TadA8e Confers Efficient and Highly Specific A-To-G Base Editing in Rice.Plant biotechnology journal · 2026Article
- Development of dual base editors based on ssDNA-targeting SCP1.201 deaminases for the artificial evolution of novel herbicide-tolerant OsEPSPS variants.Plant communications · 2026Article
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Natural variation in OsTPS8 confers differential regulation of chalkiness and seed vigor in indica and japonica rice.Nature genetics · 2026Article
- Development of cytosine and adenine base editors for maize precision breeding.Journal of integrative plant biology · 2025Article
- Fueling chromosomal gene diversification and artificial evolution with CRISPR.Genome biology · 2025Review
- Engineering Adenine Deaminase TadA for Precise and PAM-Flexible Point Mutagenesis and Gradient-Tuning Endogenous Protein Design.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- CRISPR-Cas applications in agriculture and plant research.Nature reviews. Molecular cell biology · 2025Review
- Breeding herbicide-resistant rice using CRISPR-Cas gene editing and other technologies.Plant communications · 2025Review
- Enhanced Genome Editing Activity with Novel Chimeric ScCas9 Variants in Rice.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- PhieDBEs: a DBD-containing, PAM-flexible, high-efficiency dual base editor toolbox with wide targeting scope for use in plants.Plant biotechnology journal · 2024Article
- Targeted genome-modification tools and their advanced applications in crop breeding.Nature reviews. Genetics · 2024Review
- Diverse nucleotide substitutions in rice base editing mediated by novel TadA variants.Plant communications · 2024Article
- Creating large-scale genetic diversity in Arabidopsis via base editing-mediated deep artificial evolution.Genome biology · 2024Article
- High performance TadA-8e derived cytosine and dual base editors with undetectable off-target effects in plants.Nature communications · 2024Article
Corrections and comments
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Authors and funding
18 authors at 2 institutions in 2 countries.
Funding
Abstract
CRISPR-based directed evolution is an effective breeding biotechnology to improve agronomic traits in plants. However, its gene diversification is still limited using individual single guide RNA. We described here a multiplexed orthogonal base editor (MoBE), and a randomly multiplexed sgRNAs assembly strategy to maximize gene diversification. MoBE could induce efficiently orthogonal ABE (<36.6%), CBE (<36.0%), and A&CBE (<37.6%) on different targets, while the sgRNA assembling strategy randomized base editing events on various targets. With respective 130 and 84 targets from each strand of the 34th exon of rice acetyl-coenzyme A carboxylase (OsACC), we observed the target-scaffold combination types up to 27 294 in randomly dual and randomly triple sgRNA libraries. We further performed directed evolution of OsACC using MoBE and randomly dual sgRNA libraries in rice, and obtained single or linked mutations of stronger herbicide resistance. These strategies are useful for in situ directed evolution of functional genes and may accelerate trait improvement in rice.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.