Evidence map›Paper›PMID 37570608›Full record

ArticleMolecules (Basel, Switzerland)2023

Interaction of Hoechst 33342 with POPC Membranes at Different pH Values.

Margarida M Cordeiro, Hugo A L Filipe, Patrícia Dos Santos, Jaime Samelo, João P Prates Ramalho, Luís M S Loura, Maria J Moreno

Abstract read
In one paragraph

Article in Molecules (Basel, Switzerland), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Review
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Margarida M CordeiroCoimbra Chemistry Center, Institute of Molecular Sciences (CQC-IMS), University of Coimbra, 3004-535 Coimbra, Portugal.ORCID 0000-0001-9404-831X
Hugo A L FilipeCoimbra Chemistry Center, Institute of Molecular Sciences (CQC-IMS), University of Coimbra, 3004-535 Coimbra, Portugal.ORCID 0000-0001-6935-4307
Patrícia Dos SantosCoimbra Chemistry Center, Institute of Molecular Sciences (CQC-IMS), University of Coimbra, 3004-535 Coimbra, Portugal.ORCID 0000-0002-8747-5396
Jaime SameloCoimbra Chemistry Center, Institute of Molecular Sciences (CQC-IMS), University of Coimbra, 3004-535 Coimbra, Portugal.ORCID 0000-0001-6561-9320
João P Prates RamalhoLAQV, REQUIMTE, Hercules Laboratory, Department of Chemistry, School of Science and Technology, University of Évora, 7000-671 Évora, Portugal.ORCID 0000-0003-4419-6431
Luís M S LouraCoimbra Chemistry Center, Institute of Molecular Sciences (CQC-IMS), University of Coimbra, 3004-535 Coimbra, Portugal.ORCID 0000-0002-1051-2312
Maria J MorenoCoimbra Chemistry Center, Institute of Molecular Sciences (CQC-IMS), University of Coimbra, 3004-535 Coimbra, Portugal.ORCID 0000-0003-3076-9905

Funding

Fundação para a Ciência e a Tecnologia 2022.11593.BDFundação para a Ciência e a Tecnologia UIDB/00313/2020Fundação para a Ciência e a Tecnologia UIDB/50006/2020Fundação para a Ciência e a Tecnologia UIDP/00313/2020Fundação para a Ciência e a Tecnologia UIDP/50006/2020Programa Operacional Regional do Centro CENTRO-04-3559-FSE-000162
6 · The paper itself

Abstract

Hoechst 33342 (H33342) is a fluorescent probe that is commonly used to stain the DNA of living cells. To do so, it needs to interact with and permeate through cell membranes, despite its high overall charge at physiological pH values. In this work, we address the effect of pH in the association of H33342 with lipid bilayers using a combined experimental and computational approach. The partition of H33342 to 1-palmitoyl-2-oleoyl-

Indexed as

Lipid BilayersPhosphatidylcholinesBenzimidazolesHydrogen-Ion ConcentrationMolecular Dynamics SimulationThermodynamicsBenzimidazolesbisbenzimide ethoxide trihydrochlorideLipid BilayersPhosphatidylcholinesbiomembranescationic amphiphilesfluorescent probemembrane partitionmolecular dynamics simulationsPOPC

Identifiers

PMID37570608
PMCPMC10420284

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.