Evidence map›Paper›PMID 37570330›Full record

ArticleAnimals : an open access journal from MDPI2023

Porcine Germ Cells Phenotype during Embryonic and Adult Development.

Amanda Soares Jorge, Kaiana Recchia, Mayra Hirakawa Glória, Aline Fernanda de Souza, Laís Vicari de Figueirêdo Pessôa, Paulo Fantinato Neto, Daniele Dos Santos Martins, André Furugen Cesar de Andrade, Simone Maria Massami Kitamura Martins, Fabiana Fernandes Bressan and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Animals : an open access journal from MDPI, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 2 countries.

Amanda Soares JorgeDepartment of Veterinary Medicine, School of Animal Sciences and Food Engineering, University of Sao Paulo, Pirassununga 13635-900, SP, Brazil.
Kaiana RecchiaDepartment of Surgery, Faculty of Veterinary Medicine and Animal Sciences, University of Sao Paulo, São Paulo 01001-010, SP, Brazil.ORCID 0000-0002-4697-4201
Mayra Hirakawa GlóriaDepartment of Veterinary Medicine, School of Animal Sciences and Food Engineering, University of Sao Paulo, Pirassununga 13635-900, SP, Brazil.
Aline Fernanda de SouzaDepartment Biomedical Science, Ontario Veterinary College (OVC), University of Guelph, Guelph, ON N1G 2W1, Canada.
Laís Vicari de Figueirêdo PessôaDepartment of Veterinary Medicine, School of Animal Sciences and Food Engineering, University of Sao Paulo, Pirassununga 13635-900, SP, Brazil.ORCID 0000-0002-3780-6046
Paulo Fantinato NetoDepartment of Veterinary Medicine, School of Animal Sciences and Food Engineering, University of Sao Paulo, Pirassununga 13635-900, SP, Brazil.ORCID 0000-0002-3243-8248
Daniele Dos Santos MartinsDepartment of Veterinary Medicine, School of Animal Sciences and Food Engineering, University of Sao Paulo, Pirassununga 13635-900, SP, Brazil.ORCID 0000-0002-6277-4664
André Furugen Cesar de AndradeDepartment of Animal Reproduction, Faculty of Veterinary Medicine and Animal Sciences, University of Sao Paulo, Pirassununga 13635-900, SP, Brazil.ORCID 0000-0002-0148-4372
Simone Maria Massami Kitamura MartinsDepartment of Animal Production, Faculty of Veterinary Medicine and Animal Sciences, University of São Paulo, Pirassununga 13635-900, SP, Brazil.
Fabiana Fernandes BressanDepartment of Veterinary Medicine, School of Animal Sciences and Food Engineering, University of Sao Paulo, Pirassununga 13635-900, SP, Brazil.ORCID 0000-0001-9862-5874
Naira Caroline Godoy PieriDepartment of Veterinary Medicine, School of Animal Sciences and Food Engineering, University of Sao Paulo, Pirassununga 13635-900, SP, Brazil.ORCID 0000-0002-6800-6025
Universidade de São Paulo · BRUniversity of Guelph · CA

Funding

Coordenação de Aperfeicoamento de Pessoal de Nível Superior 2017- 616; 23038.006964/2014-43 and 001São Paulo Research Foundation 2020/10503-3, 2015/25564-0, 2015/26818-5
6 · The paper itself

Abstract

Primordial germ cells (PGCs) are the precursors of gametes. Due to their importance for the formation and reproduction of an organism, understanding the mechanisms and pathways of PGCs and the differences between males and females is essential. However, there is little research in domestic animals, e.g., swine, regarding the epigenetic and pluripotency profiles of PGCs during development. This study analyzed the expression of epigenetic and various pluripotent and germline markers associated with the development and differentiation of PGCs in porcine (pPGCs), aiming to understand the different gene expression profiles between the genders. The analysis of gonads at different gestational periods (from 24 to 35 days post fertilization (dpf) and in adults) was evaluated by immunofluorescence and RT-qPCR and showed phenotypic differences between the gonads of male and female embryos. In addition, the pPGCs were positive for OCT4 and VASA; some cells were H3k27me3 positive in male embryos and adult testes. In adults, the cells of the testes were positive for germline markers, as confirmed by gene expression analysis. The results may contribute to understanding the pPGC pathways during reproductive development, while also contributing to the knowledge needed to generate mature gametes in vitro.

Indexed as

PGCsporcinereproduction

Identifiers

PMID37570330
PMCPMC10417053
OpenAlexW4385576587

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.