ArticleScientific reports2023
An evaluation of DNA extraction methods on historical and roadkill mammalian specimen.
Article in Scientific reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 20 citations in OpenAlex.
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- Electroelution Into a Salt Trap: Reviving an Old-School Approach to DNA Purification.Small methods · 2026Article
- The impact of DNA extraction methods on species quantification and apparent community composition of in vitro oral biofilms.FEMS microbiology letters · 2026Article
- Sample Preparation for Multi-Omics Analysis: Considerations and Guidance for Identifying the Ideal Workflow.Proteomics · 2025Review
- Optimizing ancient DNA recovery from archaeological plant seeds.Scientific reports · 2025Article
- A ternary-code DNA methylome atlas of mouse tissues.Genome biology · 2025Article
- A Computer Vision Method for Finding Mislabelled Specimens Within Natural History Collections.Ecology and evolution · 2025Article
- Simple DNA extraction for museum beetle specimens to unlock genetic data from historical collections.MethodsX · 2025Article
- DNA amplification from osmicated, plastic-embedded eye tissues.Molecular vision · 2025Article
- CRISPR-based strategies for sample-to-answer monkeypox detection: current status and emerging opportunities.Nanotechnology · 2024Review
- An Improved Method and Device for Nucleic Acid Isolation Using a High-Salt Gel Electroelution Trap.Analytical chemistry · 2024Article
- Isolation of High-Molecular-Weight DNA for Long-Read Sequencing Using a High-Salt Gel Electroelution Trap.Analytical chemistry · 2023Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Guidelines identifying appropriate DNA extraction methods for both museum and modern biological samples are scarce or non-existent for mammalian species. Yet, obtaining large-scale genetic material collections are vital for conservation and management purposes. In this study, we evaluated five protocols making use of either spin-column, organic solvents, or magnetic bead-based methods for DNA extraction on skin samples from both modern, traffic-killed (n = 10) and museum (n = 10) samples of European hedgehogs, Ericaneus europaeus. We showed that phenol-chloroform or silica column (NucleoSpin Tissue) protocols yielded the highest amount of DNA with satisfactory purity compared with magnetic bead-based protocols, especially for museum samples. Furthermore, extractions using the silica column protocol appeared to produce longer DNA fragments on average than the other methods tested. Our investigation demonstrates that both commercial extraction kits and phenol-chloroform protocol retrieve acceptable DNA concentrations for downstream processes, from degraded remnants of traffic-killed and museum samples of mammalian specimens. Although all the tested methods could be applied depending on the research questions and laboratory conditions, commercial extraction kits may be preferred due to their effectiveness, safety and the higher quality of the DNA extractions.
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