ArticleG3 (Bethesda, Md.)2023
Near chromosome-level genome assembly of the microsporidium Hamiltosporidium tvaerminnensis.
Article in G3 (Bethesda, Md.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
7 citing papers in PubMed, 13 citations in OpenAlex.
- Comprehensive analysis of small GTPases in Microsporidia reveals new extremes in eukaryotic cell biology.BMC biology · 2026Article
- Telomere-to-telomere genome assembly of Microsporidia sp. MB, a microsporidian symbiont of Anopheles coluzzii isolated from Burkina Faso.BMC genomics · 2026Article
- Counterproductive: coinfection of a water flea by a fungus and a microsporidium reduces the reproductive outputs of all parties.Scientific reports · 2026Article
- Variation in Genome Architecture and Epigenetic Modification Across the Microsporidia Phylogeny.Genome biology and evolution · 2025Article
- Strong Bottlenecks Constrain Adaptive Coevolution in a Host-Parasite Metapopulation.Molecular ecology · 2025Article
- De Novo Long-Read Genome Assembly and Annotation of the Mosquito Gut-Dwelling Fungus, Smittium minutisporum.Genome biology and evolution · 2024Article
- Polyploidy is widespread in Microsporidia.Microbiology spectrum · 2024Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microsporidia are intracellular parasitic fungi whose genomes rank among the smallest of all known eukaryotes. A number of outstanding questions remain concerning the evolution of their large-scale variation in genome architecture, responsible for genome size variation of more than an order of magnitude. This genome report presents the first near-chromosomal assembly of a large-genome microsporidium, Hamiltosporidium tvaerminnensis. Combined Oxford Nanopore, Pacific Biosciences (PacBio), and Illumina sequencing led to a genome assembly of 17 contigs, 11 of which represent complete chromosomes. Our assembly is 21.64 Mb in length, has an N50 of 1.44 Mb, and consists of 39.56% interspersed repeats. We introduce a novel approach in microsporidia, PacBio Iso-Seq, as part of a larger annotation pipeline for obtaining high-quality annotations of 3,573 protein-coding genes. Based on direct evidence from the full-length Iso-Seq transcripts, we present evidence for alternative polyadenylation and variation in splicing efficiency, which are potential regulation mechanisms for gene expression in microsporidia. The generated high-quality genome assembly is a necessary resource for comparative genomics that will help elucidate the evolution of genome architecture in response to intracellular parasitism.
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Registered trials
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