Evidence map›Paper›PMID 37565027›Full record

ArticleEcology and evolution2023

Library preparation method and DNA source influence endogenous DNA recovery from 100-year-old avian museum specimens.

Amie E Settlecowski, Ben D Marks, Joseph D Manthey

Open access · goldAbstract read
In one paragraph

Article in Ecology and evolution, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.5field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Amie E SettlecowskiBird Collection Gantz Family Collections Center The Field Museum Chicago Illinois USA.ORCID https://orcid.org/0000-0002-6602-0835
Ben D MarksBird Collection Gantz Family Collections Center The Field Museum Chicago Illinois USA.ORCID https://orcid.org/0009-0004-0531-1673
Joseph D MantheyDepartment of Biological Sciences Texas Tech University Lubbock Texas USA.ORCID https://orcid.org/0000-0003-2765-7611
Field Museum of Natural History · USTexas Tech University · US

Funding

Illumina NovaSeq 6000 DNA Sequencing SystemS10OD025115 · OD · TEXAS TECH UNIVERSITY · PI MECHREF, YEHIA · 2018 to 2018
$594k
NIH HHS S10 OD025115
6 · The paper itself

Abstract

Museum specimens collected prior to cryogenic tissue storage are increasingly being used as genetic resources, and though high-throughput sequencing is becoming more cost-efficient, whole genome sequencing (WGS) of historical DNA (hDNA) remains inefficient and costly due to its short fragment sizes and high loads of exogenous DNA, among other factors. It is also unclear how sequencing efficiency is influenced by DNA sources. We aimed to identify the most efficient method and DNA source for collecting WGS data from avian museum specimens. We analyzed low-coverage WGS from 60 DNA libraries prepared from four American Robin (

Indexed as

historical DNAlibrary preparationmuseomicsnatural history collectionsshotgun sequencing

Identifiers

PMID37565027
PMCPMC10410627
OpenAlexW4385700888

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.