Evidence map›Paper›PMID 37563679›Full record

ArticleInfectious diseases of poverty2023

Infection of sheep by Echinococcus multilocularis in Gansu, China: evidence from mitochondrial and nuclear DNA analysis.

Nigus Abebe Shumuye, Li Li, Wen-Hui Li, Nian-Zhang Zhang, Yan-Tao Wu, Yao-Dong Wu, Wen-Jun Tian, Lin-Sheng Zhang, Xiao-Feng Nian, Guo-Dong Dai and 9 more

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Article in Infectious diseases of poverty, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.8field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. China CDC weekly · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

19 authors at 4 institutions in 2 countries.

Nigus Abebe ShumuyeState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, National Para-Reference Laboratory for Animal Echinococcosis, Key Laboratory of Veterinary Parasitology of Gansu Province Lanzhou Veterinary Research Institute, Lanzhou University, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China.
Li LiState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, National Para-Reference Laboratory for Animal Echinococcosis, Key Laboratory of Veterinary Parasitology of Gansu Province Lanzhou Veterinary Research Institute, Lanzhou University, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China.
Wen-Hui LiState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, National Para-Reference Laboratory for Animal Echinococcosis, Key Laboratory of Veterinary Parasitology of Gansu Province Lanzhou Veterinary Research Institute, Lanzhou University, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China.
Nian-Zhang ZhangState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, National Para-Reference Laboratory for Animal Echinococcosis, Key Laboratory of Veterinary Parasitology of Gansu Province Lanzhou Veterinary Research Institute, Lanzhou University, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China.
Yan-Tao WuState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, National Para-Reference Laboratory for Animal Echinococcosis, Key Laboratory of Veterinary Parasitology of Gansu Province Lanzhou Veterinary Research Institute, Lanzhou University, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China.
Yao-Dong WuState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, National Para-Reference Laboratory for Animal Echinococcosis, Key Laboratory of Veterinary Parasitology of Gansu Province Lanzhou Veterinary Research Institute, Lanzhou University, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China.
Wen-Jun TianState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, National Para-Reference Laboratory for Animal Echinococcosis, Key Laboratory of Veterinary Parasitology of Gansu Province Lanzhou Veterinary Research Institute, Lanzhou University, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China.
Lin-Sheng ZhangState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, National Para-Reference Laboratory for Animal Echinococcosis, Key Laboratory of Veterinary Parasitology of Gansu Province Lanzhou Veterinary Research Institute, Lanzhou University, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China.
Xiao-Feng NianState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, National Para-Reference Laboratory for Animal Echinococcosis, Key Laboratory of Veterinary Parasitology of Gansu Province Lanzhou Veterinary Research Institute, Lanzhou University, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China.
Guo-Dong DaiState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, National Para-Reference Laboratory for Animal Echinococcosis, Key Laboratory of Veterinary Parasitology of Gansu Province Lanzhou Veterinary Research Institute, Lanzhou University, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China.
Wei-Gang ChenState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, National Para-Reference Laboratory for Animal Echinococcosis, Key Laboratory of Veterinary Parasitology of Gansu Province Lanzhou Veterinary Research Institute, Lanzhou University, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China.
Sheng-Zhi GaoGansu Animal Centre for Disease Control and Prevention, Lanzhou, 730046, Gansu Province, China.
Xue-Qi TianState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, National Para-Reference Laboratory for Animal Echinococcosis, Key Laboratory of Veterinary Parasitology of Gansu Province Lanzhou Veterinary Research Institute, Lanzhou University, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China.
Jun-Shi LiuJingyuan County Animal Centre for Disease Control and Prevention, Jingyuan County Animal Husbandry and Veterinary Technical Service Center, Jingyuan, 730600, Gansu Province, China.
Bin LiJingyuan County Animal Centre for Disease Control and Prevention, Jingyuan County Animal Husbandry and Veterinary Technical Service Center, Jingyuan, 730600, Gansu Province, China.
Nigatu KebedeAklilu Lemma Institute of Pathobiology, Addis Ababa University, Addis Ababa, Ethiopia.
Bao-Quan FuState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, National Para-Reference Laboratory for Animal Echinococcosis, Key Laboratory of Veterinary Parasitology of Gansu Province Lanzhou Veterinary Research Institute, Lanzhou University, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China.
Hong-Bin YanState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, National Para-Reference Laboratory for Animal Echinococcosis, Key Laboratory of Veterinary Parasitology of Gansu Province Lanzhou Veterinary Research Institute, Lanzhou University, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China. yanhongbin@caas.cn.
Wan-Zhong JiaState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, National Para-Reference Laboratory for Animal Echinococcosis, Key Laboratory of Veterinary Parasitology of Gansu Province Lanzhou Veterinary Research Institute, Lanzhou University, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China. jiawanzhong@caas.cn.ORCID http://orcid.org/0000-0002-0801-7478
Lanzhou Veterinary Research Institute · CNJingning County People's Hospital · CNAddis Ababa University · ETGansu Provincial Center for Disease Control and Prevention · CN

Funding

Central Public-Interest Scientific Institution Basal Research Fund 1610312020016Central Public-Interest Scientific Institution Basal Research Fund Y2022GH13Cultivation of Achievements of State Key Laboratory of Veterinary Etiological Biology SKLVEB2020CGPY01Gansu Provincial Major Project for Science and Technology Development 21ZD3NA001National Key Research and Development Program 2022YFC2304000National Key Research and Development Program 2022YFD1302101NBCITS CARS-37
6 · The paper itself

Abstract

backgroundIn the normal life cycle of the parasite (Echinococcus multilocularis) that causes alveolar echinococcosis, domestic and wild carnivores act as definitive hosts, and rodents act as intermediate hosts. The presented study contributes to the research on the distribution and transmission pattern of E. multilocularis in China having identified sheep as an unusual intermediate host taking part in the domestic transmission of alveolar echinococcosis in Gansu Province, China.

methodsFrom 2020 to 2021, nine whitish different cyst-like were collected from the liver of sheep in Gansu Province for examination. A near complete mitochondrial (mt) genome and selected nuclear genes were amplified from the cyst-like lesion for identification. To confirm the status of the specimen, comparative analysis with reference sequences, phylogenetic analysis, and network analysis were performed.

resultsThe isolates displayed ≥ 98.87% similarity to E. multilocularis NADH dehydrogenase sub-unit 1 (nad1) (894 bp) reference sequences deposited in GenBank. Furthermore, amplification of the nad4 and nad2 genes also confirmed all nine samples as E. multilocularis with > 99.30% similarity. Additionally, three nuclear genes, pepck (1545 bp), elp-exons VII and VIII (566 bp), and elp-exon IX (256 bp), were successfully amplified and sequenced for one of the isolates with 98.42% similarity, confirming the isolates were correctly identified as E. multilocularis. Network analysis also correctly placed the isolates with other E. multilocularis.

conclusionsAs a result of the discovery of E. multilocularis in an unusual intermediate host, which is considered to have the highest zoonotic potential, the result clearly demonstrated the necessity for expanded surveillance in the area.

Indexed as

CystsEchinococcus multilocularisAnimalsChinaDNAEchinococcosisPhylogenySheepDNAChinaEchinococcus multilocularisLiverPhylogenySheep

Identifiers

PMID37563679
PMCPMC10413491
OpenAlexW4385738503

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