Evidence map›Paper›PMID 37561186›Full record

ArticleKnee surgery, sports traumatology, arthroscopy : official journal of the ESSKA2023

Semitendinosus tendons are commonly contaminated with skin flora during graft harvest for anterior cruciate ligament reconstruction.

Christoph Offerhaus, Vera Jaecker, Sven Shafizadeh, Leonard Müller, Heiko Hahne, Hilmar Wisplinghoff, Nathalie Jazmati

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PubMed Full text at PMC Publisher
In one paragraph

Article in Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
2.5field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 8 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Christoph OfferhausDepartment of Orthopedic Surgery and Sports Traumatology, Sana Medical Centre, Witten/Herdecke University, Aachener Str. 445-449, 50933, Cologne, Germany. christoph.offerhaus@uni-wh.de.ORCID http://orcid.org/0000-0003-2711-6559
Vera JaeckerDepartment of Trauma Surgery, Orthopaedic Surgery and Sports Traumatology, Cologne Merheim Medical Centre, Witten/Herdecke University, Cologne, Germany.
Sven ShafizadehDepartment of Orthopedic Surgery and Sports Traumatology, Sana Medical Centre, Witten/Herdecke University, Aachener Str. 445-449, 50933, Cologne, Germany.
Leonard MüllerFaculty of Medicine, University of Cologne, Cologne, Germany.
Heiko HahneFaculty of Medicine, University of Cologne, Cologne, Germany.
Hilmar WisplinghoffWisplinghoff Laboratories, Cologne, Germany.
Nathalie JazmatiWisplinghoff Laboratories, Cologne, Germany.
Witten/Herdecke University · DEUniversity of Cologne · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeTo investigate the rate of bacterial contamination of semitendinosus (ST) tendons during graft harvest in anterior cruciate ligament reconstruction (ACLR), in order to precisely specify the underlying pathogens and obtain data on their susceptibility to potential antibiotics.

methodsIn a prospective study, a total of 59 consecutive patients undergoing primary ACLR were recruited from one centre. No patient had history of previous surgery to the knee or showed clinical signs of infection. Four tissue samples of harvested ST tendons for anterior cruciate ligament (ACL) autografts (case group; ST) were examined for evidence of bacterial colonisation and compared to four tissue samples of the native ACL as negative controls (control group; ACL). Three of the respective samples were subjected to cultural microbiological examination and one to 16S rRNA-PCR. Antibiotic susceptibility testing was performed for each pathogen that was identified.

resultsA total of 342 samples were analysed by culture. Significantly more patients showed a positive culture of the ST (33.9%; n = 20/59) compared to 3.4% of patients (n = 2/59) with positive culturing of the ACL (p < 0.0001). Including 16S rRNA-PCR, in a total of 42.4% (25/59) of patients, bacteria were detected in at least one ST sample either by PCR and/or culture. All species found (n = 33) belong to the typical skin flora with Staphylococcus epidermidis (39.4%; n = 13/33) being the most common species, followed by Staphylococcus capitis (24.2%; n = 8/33). All tested isolates (n = 29) were susceptible to vancomycin (29/29, 100%), 69% (n = 20/29) to oxacillin and 65.5% (n = 19/29) to clindamycin.

conclusionST autografts for ACLR were commonly contaminated with skin commensal bacteria during harvest. One-third of the isolates showed resistance to typical perioperative intravenous antibiotics, whereas all isolates were sensitive to vancomycin. Therefore, routine prophylactic decontamination of all hamstring autografts before implantation should be recommended, preferably with topical vancomycin. LEVEL OF EVIDENCE: Level III.

Indexed as

Anterior Cruciate Ligament InjuriesAnterior Cruciate Ligament ReconstructionHamstring TendonsAnti-Bacterial AgentsAutograftsHumansProspective StudiesRNA, Ribosomal, 16SVancomycinAnti-Bacterial AgentsRNA, Ribosomal, 16SVancomycinACL contaminationAnterior cruciate ligamentAntibiotic resistanceGraft harvestInfection preventionSemitendinosusVancomycin

Identifiers

PMID37561186
PMCPMC10598102
OpenAlexW4385716735

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.