ArticleThe veterinary quarterly2023
Quantification of GS-441524 concentration in feline plasma using high performance liquid chromatography with fluorescence detection.
Article in The veterinary quarterly, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 7 citations in OpenAlex.
- A Validated Eco-Friendly High-Performance Liquid Chromatography Assay for Therapeutic Drug Monitoring of GS-441524 in Cats with Feline Infectious Peritonitis.Pathogens (Basel, Switzerland) · 2026Article
- Development and Physicochemical Stability of an Oral GS-441524 Suspension for Weight-Adjusted Treatment of Feline Infectious Peritonitis.Veterinary medicine and science · 2026Article
- LC-MS/MS Therapeutic Drug Monitoring of GS-441524 in Serum and Various Compounded Formulations to Improve the Treatment of Feline Infectious Peritonitis.Animals : an open access journal from MDPI · 2026Article
- Epidemiological and Clinical Insights from 68 Veterinarian-Reported Cases of Feline Infectious Peritonitis During the Documented FIP Epizootic in Cyprus.Pathogens (Basel, Switzerland) · 2026Article
- Therapeutic Drug Monitoring of GS-441524 in Cats with Feline Infectious Peritonitis: Pharmacokinetic Variability and Implications for Dose Optimization.Pathogens (Basel, Switzerland) · 2026Article
- Clinical therapeutics in feline medicine: updates for old and new drugs.Journal of feline medicine and surgery · 2025Review
- Pharmacokinetics of GS-441524 following intravenous remdesivir in six cats and results of therapeutic drug monitoring during treatment of feline infectious peritonitis: 22 cases (2021-2024).The Journal of small animal practice · 2025Article
- Efficacy of oral remdesivir in treating feline infectious peritonitis: a prospective observational study of 29 cats.Journal of feline medicine and surgery · 2025Article
- AssessingThe veterinary quarterly · 2024Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The adenosine analogue GS-441524 has demonstrated efficacy in treatment of feline infectious peritonitis (FIP). With no commercially registered formulations of GS-441524 available, global focus shifted to its pro-drug remdesivir, as it became more accessible throughout the COVID-19 pandemic. This study developed and validated a simple liquid chromatography equipped with a fluorescence detector to quantify plasma concentrations of GS-441524 applicable for routine therapeutic monitoring of remdesivir or GS-441524 therapy for FIP infected cats. A Waters X-Bridge C18, 5 µm, 150 × 4.6 mm, column was used and mixtures of 20 mM ammonium acetate (pH 4.5) with acetonitrile of 5% and 70% were prepared for gradient mobile phase. With a simple protein precipitation using methanol to clean plasma sample, GS-441524 was monitored at excitation and emission wavelengths of 250 nm and 475 nm, respectively. Using an external standard, the lowest and highest limits of quantification were 19.5 ng/mL to 10,000 ng/mL, respectively. The intra- and inter day trueness of the quality controls (QCs) were within 10% of their nominal concentrations and intra- and inter day precision of the QCs (expressed as the coefficient of variation) ranged from 1.7 to 5.7%, This assay was able to quantify plasma trough levels of GS-441524 (23.7-190.1 ng/mL) after the administration of remdesivir (9.9-15.0 mg/kg BW, IV or SC) in FIP cats (
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