Evidence map›Paper›PMID 37554427›Full record

ArticleBiochemistry and biophysics reports2023

NMR indicates the N-termini of PSGL1 and CCR7 bind competitively to the chemokine CCL21.

Robin N Witt, Kaileigh S Nickel, John R Binns, Alexander M Gray, Alyssa M Hintz, Noah F Kofron, Steven F Steigleder, Francis C Peterson, Christopher T Veldkamp

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biochemistry and biophysics reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Robin N WittDepartment of Chemistry, University of Wisconsin-Whitewater, 800 West Main Street, Whitewater, WI, 53190, USA.
Kaileigh S NickelDepartment of Chemistry, University of Wisconsin-Whitewater, 800 West Main Street, Whitewater, WI, 53190, USA.
John R BinnsDepartment of Chemistry, University of Wisconsin-Whitewater, 800 West Main Street, Whitewater, WI, 53190, USA.
Alexander M GrayDepartment of Chemistry, University of Wisconsin-Whitewater, 800 West Main Street, Whitewater, WI, 53190, USA.
Alyssa M HintzDepartment of Chemistry, University of Wisconsin-Whitewater, 800 West Main Street, Whitewater, WI, 53190, USA.
Noah F KofronDepartment of Biology, University of Wisconsin-Whitewater, 800 West Main Street, Whitewater, WI, 53190, USA.
Steven F SteiglederDepartment of Chemistry, University of Wisconsin-Whitewater, 800 West Main Street, Whitewater, WI, 53190, USA.
Francis C PetersonDepartment of Biochemistry, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI, 53226, USA.
Christopher T VeldkampDepartment of Chemistry, University of Wisconsin-Whitewater, 800 West Main Street, Whitewater, WI, 53190, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Chemokines are from a family of secreted cytokines that direct the trafficking of immune cells to coordinate immune responses. Chemokines are involved in numerous disease states, including responding to infections, autoimmune disorders, and cancer metastasis. Ther are chemokines, like CCL21, that signal for cellular migration through the activation of G protein-coupled receptors, like CCR7, through interaction with the receptor's extracellular N-terminus, loops, and core of the receptor. CCL21 is involved in routine immune surveillance but can also attract metastasizing cancer cells to lymph nodes. P-selectin glycoprotein ligand 1 (PSGL1) has a role in cellular adhesion during chemotaxis and is a transmembrane signaling molecule. PSGL1 expression enhances chemotactic responses of T cells to CCL21. Here NMR studies indicate the binding sites on CCL21 for the N-termini or PSGL1 and CCR7 overlap, and binding of the N-termini of PSGL1 and CCR7 is competitive.

Indexed as

CCL21CCR7Chemical shift mappingChemokinesCompetitive bindingMetastasisNMRPSGL1

Identifiers

PMID37554427
PMCPMC10404610

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.