ArticleNature communications2023
spinDrop: a droplet microfluidic platform to maximise single-cell sequencing information content.
Article in Nature communications, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed.
- Construction of mannosylated glycol-nanoparticles for near-infrared bioimaging and photodynamic therapy targeting breast cancer cells.RSC advances · 2026Article
- Protocol for isolation of nuclei from murine cardiac tissue for single-nucleus multiomic sequencing.STAR protocols · 2026Article
- Integrated microfluidic biosensors: shaping the future of quantitative life sciences and on-chip molecular diagnostics.Lab on a chip · 2026Review
- Intelligent label-free droplet microfluidic sorting system for single-cell encapsulation and morphology-guided screening.Microsystems & nanoengineering · 2026Article
- BenchDrop-seq: a microfluidics-free platform for benchtop single-cell long-read RNA sequencing.bioRxiv : the preprint server for biology · 2026Article
- AI-Based Prediction of Gene Expression in Single-Cell and Multiscale Genomics and Transcriptomics.International journal of molecular sciences · 2026Review
- Article
- Droplet-based single-cell RNA sequencing: decoding cellular heterogeneity for breakthroughs in cancer, reproduction, and beyond.Journal of translational medicine · 2025Review
- Single-cell sequencing technology in renal cancer: insights into tumor biology and clinical application.Biomarker research · 2025Review
- Cap-Drop: A Pre-Programmed, Self-Powered Capillary Microfluidic System for Passive Droplet Generation and 3D Cell Culture Modeling.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- An acoustofluidic embedding platform for rapid multiphase microparticle injection.Nature communications · 2025Article
- From specialization to broad adoption: Key trends in droplet microfluidic innovations enhancing accessibility to non-experts.Biomicrofluidics · 2025Article
- inDrops-2: a flexible, versatile and cost-efficient droplet microfluidic approach for high-throughput scRNA-seq of fresh and preserved clinical samples.Nucleic acids research · 2025Article
- Isolation and Enumeration of Circulating Endothelial Cells with Antibody-Labeled Staggered Herringbone Microfluidic System.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Multiplets in scRNA-seq data: Extent of the problem and efficacy of methods for removal.PloS one · 2025Article
- Advancements in single-cell RNA sequencing and spatial transcriptomics: transforming biomedical research.Acta biochimica Polonica · 2025Review
- scTrends: A living review of commercial single-cell and spatial 'omic technologies.Cell genomics · 2024Review
- An Acoustofluidic Picoinjector.Sensors and actuators. B, Chemical · 2024Article
- DropBlot: single-cell western blotting of chemically fixed cancer cells.Nature communications · 2024Article
- CellRank 2: unified fate mapping in multiview single-cell data.Nature methods · 2024Article
Corrections and comments
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Authors and funding
12 authors.
Funding
Abstract
Droplet microfluidic methods have massively increased the throughput of single-cell sequencing campaigns. The benefit of scale-up is, however, accompanied by increased background noise when processing challenging samples and the overall RNA capture efficiency is lower. These drawbacks stem from the lack of strategies to enrich for high-quality material or specific cell types at the moment of cell encapsulation and the absence of implementable multi-step enzymatic processes that increase capture. Here we alleviate both bottlenecks using fluorescence-activated droplet sorting to enrich for droplets that contain single viable cells, intact nuclei, fixed cells or target cell types and use reagent addition to droplets by picoinjection to perform multi-step lysis and reverse transcription. Our methodology increases gene detection rates fivefold, while reducing background noise by up to half. We harness these properties to deliver a high-quality molecular atlas of mouse brain development, despite starting with highly damaged input material, and provide an atlas of nascent RNA transcription during mouse organogenesis. Our method is broadly applicable to other droplet-based workflows to deliver sensitive and accurate single-cell profiling at a reduced cost.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.