Evidence map›Paper›PMID 37552374›Full record

ArticleAAPS PharmSciTech2023

A Bacteriophage-Loaded Microparticle Laden Topical Gel for the Treatment of Multidrug-Resistant Biofilm-Mediated Burn Wound Infection.

Deepa Dehari, Aiswarya Chaudhuri, Dulla Naveen Kumar, Meraj Anjum, Rajesh Kumar, Akshay Kumar, Dinesh Kumar, Gopal Nath, Ashish Kumar Agrawal

Abstract read
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In one paragraph

Article in AAPS PharmSciTech, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
2.7field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 11 citations in OpenAlex.

  1. Review
  2. Review
  3. Microbiotherapy: an emerging adjunct for burn wound healing.Frontiers in cellular and infection microbiology · 2026
    Review
  4. Application of three-dimensional bacteriophage cocktail biogel on infected burn wounds in rats.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2025
    Article
  5. Article
  6. Review
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Deepa DehariDepartment of Pharmaceutical Engineering and Technology, Indian Institute of Technology (BHU), Varanasi, U.P., 221005, India.
Aiswarya ChaudhuriDepartment of Pharmaceutical Engineering and Technology, Indian Institute of Technology (BHU), Varanasi, U.P., 221005, India.
Dulla Naveen KumarDepartment of Pharmaceutical Engineering and Technology, Indian Institute of Technology (BHU), Varanasi, U.P., 221005, India.
Meraj AnjumDepartment of Pharmaceutical Engineering and Technology, Indian Institute of Technology (BHU), Varanasi, U.P., 221005, India.
Rajesh KumarDepartment of Microbiology, Institute of Medical Science, Banaras Hindu University, Varanasi, U.P., 221005, India.
Akshay KumarDepartment of Microbiology, Institute of Medical Science, Banaras Hindu University, Varanasi, U.P., 221005, India.
Dinesh KumarDepartment of Pharmaceutical Engineering and Technology, Indian Institute of Technology (BHU), Varanasi, U.P., 221005, India.
Gopal NathDepartment of Microbiology, Institute of Medical Science, Banaras Hindu University, Varanasi, U.P., 221005, India.
Ashish Kumar AgrawalDepartment of Pharmaceutical Engineering and Technology, Indian Institute of Technology (BHU), Varanasi, U.P., 221005, India. ashish.phe@iitbhu.ac.in.ORCID http://orcid.org/0000-0002-7302-3647
Indian Institute of Technology BHU · INBanaras Hindu University · IN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Klebsiella pneumoniae is regarded as one of the most profound bacteria isolated from the debilitating injuries caused by burn wounds. In addition, the multidrug resistance (MDR) and biofilm formation make treating burn patients with clinically available antibiotics difficult. Bacteriophage therapy has been proven an effective alternative against biofilm-mediated wound infections caused by MDR bacterial strains. In the current study, the bacteriophage (BPKPФ1) against MDR Klebsiella pneumoniae was isolated and loaded into the chitosan microparticles (CHMPs), which was later incorporated into the Sepineo P 600 to convert into a gel (BPKPФ1-CHMP-gel). BPKPФ1 was characterized for lytic profile, morphological class, and burst size, which revealed that the BPKPФ1 belongs to the family Siphoviridae. Moreover, BPKPФ1 exhibited a narrow host range with 128 PFU/host cell of burst size. The BPKPФ1-loaded CHMPs showed an average particle size of  1.96 ± 0.51 μm, zeta potential 32.16 ± 0.41 mV, and entrapment efficiency in the range of 82.44 ± 1.31%. Further, the in vitro antibacterial and antibiofilm effectiveness of BPKPФ1-CHMPs-gel were examined. The in vivo potential of the BPKPФ1-CHMPs-gel was assessed using a rat model with MDR Klebsiella pneumoniae infected burn wound, which exhibited improved wound contraction (89.22 ± 0.48%) in 28 days with reduced inflammation, in comparison with different controls. Data in hand suggest the potential of bacteriophage therapy to be developed as personalized therapy in case of difficult-to-treat bacterial infections.

Indexed as

BacteriophagesBurnsChitosanKlebsiella InfectionsWound InfectionAnimalsAnti-Bacterial AgentsBiofilmsGelsKlebsiella pneumoniaeRatsAnti-Bacterial AgentsChitosanGelsbacterial infectionsbacteriophage formulationchitosan microparticlesklebsiella pneumoniaewound healing

Identifiers

PMID37552374
OpenAlexW4385660244

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.