ArticleAsian biomedicine : research, reviews and news2020
Production and differential activity of recombinant human wild-type G6PD and G6PD
Article in Asian biomedicine : research, reviews and news, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Background: Glucose-6-phosphate dehydrogenase (G6PD) is essential to produce reduced nicotinamide adenine dinucleotide phosphate, which is required to protect cells against oxidative stress. G6PD deficiency is a genetic variation that may lead to hemolysis with potential consequences, such as kidney failure, and patients often experience low quality of life. Objectives: To establish a simple, efficient, and optimized method to produce a G6PD Methods: Results: Both amplicons were successfully cloned into a pET26b(+) expression vector and transformed into Conclusions: We successfully cloned, expressed, and purified both wild-type G6PD and G6PD
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