Evidence map›Paper›PMID 37550723›Full record

ArticleInfectious diseases of poverty2023

Optimisation of the DNA dipstick as a rapid extraction method for Schistosoma japonicum in infected mice samples and spiked human clinical samples.

Oyime P Aula, Donald P McManus, Malcolm K Jones, Hong You, Pengfei Cai, Catherine A Gordon

Abstract read
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Article in Infectious diseases of poverty, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 1 synthesis or guideline pooled it.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Oyime P AulaMolecular Parasitology Laboratory, QIMR Berghofer Medical Research Institute, Brisbane, Australia. Oyime.aula@qimrberghofer.edu.au.ORCID http://orcid.org/0000-0001-8746-4796
Donald P McManusMolecular Parasitology Laboratory, QIMR Berghofer Medical Research Institute, Brisbane, Australia.
Malcolm K JonesMolecular Parasitology Laboratory, QIMR Berghofer Medical Research Institute, Brisbane, Australia.
Hong YouMolecular Parasitology Laboratory, QIMR Berghofer Medical Research Institute, Brisbane, Australia.
Pengfei CaiMolecular Parasitology Laboratory, QIMR Berghofer Medical Research Institute, Brisbane, Australia.
Catherine A GordonMolecular Parasitology Laboratory, QIMR Berghofer Medical Research Institute, Brisbane, Australia. Catherine.gordon@qimrberghofer.edu.au.

Funding

National Health and Medical Research Council APP1098244National Health and Medical Research Council APP1102926National Health and Medical Research Council APP1132975National Health and Medical Research Council APP1160046National Health and Medical Research Council APP1194462National Health and Medical Research Council APP2008433
6 · The paper itself

Abstract

backgroundSchistosomiasis remains a public health issue and the need for accurate and affordable diagnostics is crucial in the elimination of the disease. While molecular diagnostics are highly effective, they are expensive, with the main costs been associated with DNA extraction. The DNA dipstick is a rapid, affordable and simple purification method that allows DNA to be extracted from diagnostic samples within 30 s. We aimed to optimise the DNA dipstick method for samples from mice and egg-spiked human samples.

methodsUrine, blood and faeces were collected from mice exposed to Schistosoma japonicum infection at weekly intervals from Day 0 to Day 42. Urine and faecal samples were also collected from volunteer, uninfected humans and spiked with S. japonicum eggs. All samples were subject to several optimisation procedures and DNA extracted with the DNA dipstick. Amplification of the target DNA was carried out using LAMP and visualised using agarose gel electrophoresis and flocculation.

resultsThe DNA dipstick successfully identified S. japonicum from infected mice and human clinical samples spiked with cracked eggs or genomic DNA from S. japonicum. Amplification was observed from week 4 post infection in infected mice. For human samples, amplification was observed in sieved faecal samples, filtered urine samples heated at 95 °C for 30 min, and sera samples heated at 95 °C for 30 min.

conclusionsThe DNA dipstick combined with LAMP has huge potential in providing cost-effective, simple and accurate detection of schistosomiasis infection in endemic regions. This will allow for rapid treatment, tracking outbreaks-such as occur after typhoons, leading to better health outcomes and contributing to control and eventual elimination of schistosomiasis.

Indexed as

Schistosoma japonicumSchistosomiasisSchistosomiasis japonicaAnimalsDNA, HelminthHumansMiceNucleic Acid Amplification TechniquesSensitivity and SpecificityDNA, HelminthDNA dipstickFlocculationLoop-mediated isothermal amplification assaySchistosoma japonicumSchistosomiasis

Identifiers

PMID37550723
PMCPMC10405380

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.