Evidence map›Paper›PMID 37550295›Full record

ArticleNature communications2023

Cas9-mediated knockout of Ndrg2 enhances the regenerative potential of dendritic cells for wound healing.

Dominic Henn, Dehua Zhao, Dharshan Sivaraj, Artem Trotsyuk, Clark Andrew Bonham, Katharina S Fischer, Tim Kehl, Tobias Fehlmann, Autumn H Greco, Hudson C Kussie and 12 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
5.7field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 37 citations in OpenAlex.

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  14. Apoptosis and Cell Clearance in Skin Wound Healing.Advances in experimental medicine and biology · 2025
    Review
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  18. Dendritic Cell-Associated MARCKSL1 Regulates Fibroblast Differentiation During Wound Healing.Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

22 authors at 6 institutions in 2 countries.

Dominic HennHagey Laboratory for Pediatric Regenerative Medicine, Division of Plastic and Reconstructive Surgery, Stanford University, Stanford, CA, USA.
Dehua Zhao *Department of Bioengineering, Sarafan ChEM-H, Stanford University, Stanford, CA, USA.
Dharshan Sivaraj *Hagey Laboratory for Pediatric Regenerative Medicine, Division of Plastic and Reconstructive Surgery, Stanford University, Stanford, CA, USA.ORCID http://orcid.org/0000-0001-7453-0045
Artem TrotsyukHagey Laboratory for Pediatric Regenerative Medicine, Division of Plastic and Reconstructive Surgery, Stanford University, Stanford, CA, USA.
Clark Andrew BonhamHagey Laboratory for Pediatric Regenerative Medicine, Division of Plastic and Reconstructive Surgery, Stanford University, Stanford, CA, USA.
Katharina S FischerHagey Laboratory for Pediatric Regenerative Medicine, Division of Plastic and Reconstructive Surgery, Stanford University, Stanford, CA, USA.
Tim KehlCenter for Bioinformatics, Saarland Informatics Campus, Saarland University, Saarbrücken, Germany.ORCID http://orcid.org/0000-0002-9523-7566
Tobias FehlmannChair for Clinical Bioinformatics, Saarland University, Saarbruecken, Germany.ORCID http://orcid.org/0000-0003-1967-2918
Autumn H GrecoHagey Laboratory for Pediatric Regenerative Medicine, Division of Plastic and Reconstructive Surgery, Stanford University, Stanford, CA, USA.ORCID http://orcid.org/0000-0002-7557-6800
Hudson C KussieHagey Laboratory for Pediatric Regenerative Medicine, Division of Plastic and Reconstructive Surgery, Stanford University, Stanford, CA, USA.
Sylvia E Moortgat IllouzHagey Laboratory for Pediatric Regenerative Medicine, Division of Plastic and Reconstructive Surgery, Stanford University, Stanford, CA, USA.
Jagannath PadmanabhanHagey Laboratory for Pediatric Regenerative Medicine, Division of Plastic and Reconstructive Surgery, Stanford University, Stanford, CA, USA.ORCID http://orcid.org/0000-0003-1096-6637
Janos A BarreraHagey Laboratory for Pediatric Regenerative Medicine, Division of Plastic and Reconstructive Surgery, Stanford University, Stanford, CA, USA.ORCID http://orcid.org/0000-0001-6811-8337
Ulrich KneserDepartment of Hand, Plastic, and Reconstructive Surgery, BG Trauma Center Ludwigshafen, Ruprecht-Karls-University of Heidelberg, Heidelberg, Germany.
Hans-Peter LenhofCenter for Bioinformatics, Saarland Informatics Campus, Saarland University, Saarbrücken, Germany.
Michael JanuszykHagey Laboratory for Pediatric Regenerative Medicine, Division of Plastic and Reconstructive Surgery, Stanford University, Stanford, CA, USA.
Benjamin LeviDepartment of Burn, Trauma, Acute and Critical Care Surgery, University of Texas Southwestern Medical Center, Dallas, TX, USA.
Andreas KellerCenter for Bioinformatics, Saarland Informatics Campus, Saarland University, Saarbrücken, Germany.ORCID http://orcid.org/0000-0002-5361-0895
Michael T LongakerHagey Laboratory for Pediatric Regenerative Medicine, Division of Plastic and Reconstructive Surgery, Stanford University, Stanford, CA, USA.
Kellen ChenHagey Laboratory for Pediatric Regenerative Medicine, Division of Plastic and Reconstructive Surgery, Stanford University, Stanford, CA, USA.ORCID http://orcid.org/0000-0002-7023-7850
Lei S QiDepartment of Bioengineering, Sarafan ChEM-H, Stanford University, Stanford, CA, USA. slqi@stanford.edu.ORCID http://orcid.org/0000-0002-3965-3223
Geoffrey C GurtnerHagey Laboratory for Pediatric Regenerative Medicine, Division of Plastic and Reconstructive Surgery, Stanford University, Stanford, CA, USA. ggurtner@stanford.edu.ORCID http://orcid.org/0009-0002-0706-8035
Stanford University · USUniversity of Arizona · USSaarland University · DEThe University of Texas Southwestern Medical Center · USChan Zuckerberg Initiative (United States) · USHeidelberg University · DE

Funding

Stanford Islet Research CoreP30DK116074 · NIDDK · STANFORD UNIVERSITY · PI Seung K Kim · 2017 to 2026
$19.5M
Reversal of Progenitor Cell Deficits in DiabetesR01DK074095 · NIDDK · UNIVERSITY OF ARIZONA · PI GURTNER, GEOFFREY C · 2005 to 2025
$6.4M
Ultra high throughput sequencer for sustaining and enhancing multi-scale genomic studiesS10OD025212 · OD · STANFORD UNIVERSITY · PI SNYDER, MICHAEL P. · 2018 to 2018
$600k
High Throughput DNA Sequencer UpgradeS10OD018220 · OD · STANFORD UNIVERSITY · PI COLLER, JOHN · 2014 to 2014
$596k
Computer ClusterS10OD021763 · OD · STANFORD UNIVERSITY · PI JI, HANLEE P · 2016 to 2016
$481k
Probing relationships between DNA methylation and cellular senescence with high-throughput CRISPR-based epigenetic editingR21AG077193 · NIA · STANFORD UNIVERSITY · PI QI, LEI STANLEY · 2023 to 2024
$425k
NIA NIH HHS R21 AG077193NIDDK NIH HHS P30 DK116074NIDDK NIH HHS R01 DK074095NIH HHS S10 OD018220NIH HHS S10 OD021763NIH HHS S10 OD025212
6 · The paper itself

Abstract

Chronic wounds impose a significant healthcare burden to a broad patient population. Cell-based therapies, while having shown benefits for the treatment of chronic wounds, have not yet achieved widespread adoption into clinical practice. We developed a CRISPR/Cas9 approach to precisely edit murine dendritic cells to enhance their therapeutic potential for healing chronic wounds. Using single-cell RNA sequencing of tolerogenic dendritic cells, we identified N-myc downregulated gene 2 (Ndrg2), which marks a specific population of dendritic cell progenitors, as a promising target for CRISPR knockout. Ndrg2-knockout alters the transcriptomic profile of dendritic cells and preserves an immature cell state with a strong pro-angiogenic and regenerative capacity. We then incorporated our CRISPR-based cell engineering within a therapeutic hydrogel for in vivo cell delivery and developed an effective translational approach for dendritic cell-based immunotherapy that accelerated healing of full-thickness wounds in both non-diabetic and diabetic mouse models. These findings could open the door to future clinical trials using safe gene editing in dendritic cells for treating various types of chronic wounds.

Indexed as

Craniocerebral TraumaCRISPR-Cas SystemsAnimalsDendritic CellsGene EditingGenes, mycHumansMiceWound Healing

Identifiers

PMID37550295
PMCPMC10406832
OpenAlexW4385636384

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.