Evidence map›Paper›PMID 37550278›Full record

ArticleNature communications2023

Phenotypically complex living materials containing engineered cyanobacteria.

Debika Datta, Elliot L Weiss, Daniel Wangpraseurt, Erica Hild, Shaochen Chen, James W Golden, Susan S Golden, Jonathan K Pokorski

Abstract read
In one paragraph

Article in Nature communications, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Review
  9. Review
  10. A responsive living material prepared by diffusion reveals extracellular enzyme activity of cyanobacteria.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  11. Review
  12. Article
  13. Article
  14. Review
  15. Article
  16. Review
  17. Sonosynthetic Cyanobacteria Oxygenation for Self-Enhanced Tumor-Specific Treatment.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024
    Article
  18. Article
  19. Light management by algal aggregates in living photosynthetic hydrogels.Proceedings of the National Academy of Sciences of the United States of America · 2024
    Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Debika Datta *Department of Nanoengineering, University of California San Diego, La Jolla, CA, USA.ORCID http://orcid.org/0000-0001-7146-7095
Elliot L Weiss *Scripps Institution of Oceanography, University of California San Diego, La Jolla, CA, USA.ORCID http://orcid.org/0000-0002-7713-6198
Daniel WangpraseurtDepartment of Nanoengineering, University of California San Diego, La Jolla, CA, USA.ORCID http://orcid.org/0000-0003-4834-8981
Erica HildDepartment of Nanoengineering, University of California San Diego, La Jolla, CA, USA.
Shaochen ChenDepartment of Nanoengineering, University of California San Diego, La Jolla, CA, USA.ORCID http://orcid.org/0000-0001-6876-497X
James W GoldenDepartment of Molecular Biology, University of California San Diego, La Jolla, CA, USA.ORCID http://orcid.org/0000-0001-5463-3207
Susan S GoldenDepartment of Molecular Biology, University of California San Diego, La Jolla, CA, USA. sgolden@ucsd.edu.ORCID http://orcid.org/0000-0002-4264-7019
Jonathan K PokorskiDepartment of Nanoengineering, University of California San Diego, La Jolla, CA, USA. jpokorski@ucsd.edu.ORCID http://orcid.org/0000-0001-5869-6942

Funding

U of Calif, San Diego Neuroscience Microscopy ImagingP30NS047101 · NINDS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI GLEESON, JOSEPH G, ZHENG, BINHAI · 2003 to 2022
$9.0M
NINDS NIH HHS P30 NS047101
6 · The paper itself

Abstract

The field of engineered living materials lies at the intersection of materials science and synthetic biology with the aim of developing materials that can sense and respond to the environment. In this study, we use 3D printing to fabricate a cyanobacterial biocomposite material capable of producing multiple functional outputs in response to an external chemical stimulus and demonstrate the advantages of utilizing additive manufacturing techniques in controlling the shape of the fabricated photosynthetic material. As an initial proof-of-concept, a synthetic riboswitch is used to regulate the expression of a yellow fluorescent protein reporter in Synechococcus elongatus PCC 7942 within a hydrogel matrix. Subsequently, a strain of S. elongatus is engineered to produce an oxidative laccase enzyme; when printed within a hydrogel matrix the responsive biomaterial can decolorize a common textile dye pollutant, indigo carmine, potentially serving as a tool in environmental bioremediation. Finally, cells are engineered for inducible cell death to eliminate their presence once their activity is no longer required, which is an important function for biocontainment and minimizing environmental impact. By integrating genetically engineered stimuli-responsive cyanobacteria in volumetric 3D-printed designs, we demonstrate programmable photosynthetic biocomposite materials capable of producing functional outputs including, but not limited to, bioremediation.

Indexed as

SynechococcusBacterial ProteinsHydrogelsMetabolic EngineeringPhotosynthesisSynthetic BiologyBacterial ProteinsHydrogels

Identifiers

PMID37550278
PMCPMC10406891

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.