Evidence map›Paper›PMID 37549633›Full record

ArticleDiagnostic microbiology and infectious disease2023

Reliability of nasopharyngeal PCR for the detection of otopathogens in children with uncomplicated acute otitis media compared to culture.

Holly M Frost, Timothy C Jenkins, Thresia Sebastian, Sarah K Parker, Amy Keith, Melanie Kurtz, Dana R Fletcher, Michael L Wilson, Samuel R Dominguez

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Diagnostic microbiology and infectious disease, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.3field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Article
  3. New insights into the treatment of acute otitis media.Expert review of anti-infective therapy · 2023
    Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Holly M FrostDepartment of Pediatrics, Denver Health and Hospital Authority, Denver, CO, USA; Center for Health Systems Research, Denver Health and Hospital Authority, Denver, CO, USA; Department of Pediatrics, University of Colorado School of Medicine, Aurora, CO, USA. Electronic address: Holly.Frost@dhha.org.
Timothy C JenkinsDivision of Infectious Diseases, Department of Medicine, Denver Health and Hospital Authority, Denver, CO, USA; Division of Infectious Diseases, Department of Medicine, University of Colorado School of Medicine, Aurora, CO, USA.
Thresia SebastianDepartment of Pediatrics, Denver Health and Hospital Authority, Denver, CO, USA; Department of Pediatrics, University of Colorado School of Medicine, Aurora, CO, USA; Department of Pediatrics, Alameda Health System, Oakland, CA, USA.
Sarah K ParkerDepartment of Pediatric Infectious Diseases, University of Colorado School of Medicine, Aurora, CO, USA; Department of Pediatric Infectious Diseases, Children's Hospital Colorado, Aurora, CO, USA.
Amy KeithCenter for Health Systems Research, Denver Health and Hospital Authority, Denver, CO, USA.
Melanie KurtzCenter for Health Systems Research, Denver Health and Hospital Authority, Denver, CO, USA.
Dana R FletcherEvida Research Consulting, Denver, CO, USA.
Michael L WilsonDepartment of Pathology and Laboratory Services, Denver Health and Hospital Authority, Denver, CO, USA; Department of Pathology, University of Colorado School of Medicine, Aurora, CO, USA.
Samuel R DominguezDepartment of Pediatric Infectious Diseases, University of Colorado School of Medicine, Aurora, CO, USA; Department of Pediatric Infectious Diseases, Children's Hospital Colorado, Aurora, CO, USA.
Denver Health Medical Center · USUniversity of Colorado Hospital · USChildren's Hospital Colorado · US

Funding

Rapid diagnostic tests for adenovirus conjunctivitis in children: An epidemiologic, clinical, and cost-effectiveness evaluationK23HD099925 · NICHD · DENVER HEALTH AND HOSPITAL AUTHORITY · PI FROST, HOLLY · 2019 to 2023
$762k
NICHD NIH HHS K23 HD099925
6 · The paper itself

Abstract

Otopathogens in acute otitis media (AOM) have implications for care because the likelihood of resolution without antibiotics and optimal antibiotic agent varies by microorganism. We aimed to determine the sensitivity, specificity, positive predictive value, and negative predictive value of nasopharyngeal (NP) qualitative polymerase chain reaction (PCR) for common bacterial otopathogens in children with AOM compared to NP culture. NP flocked swabs collected from enrolled children aged 6 to 35 months with uncomplicated AOM in Denver, CO were tested by culture and multiplex PCR. The sensitivity and negative predictive value of PCR using culture as a reference were high (H. influenzae 93.3%, 98.0%; S. pneumoniae 94.2%, 95.1%; M. catarrhalis 92.3%, 86.4%); whereas the specificity and positive predictive value were lower and varied by organism (54.2%-84.1%, 55.1%-69.2%, respectively). PCR detected 1.5 times more organisms than culture. NP PCR has a high predictive value for excluding otopathogens compared to culture and warrants exploration as a diagnostic tool.

Indexed as

Moraxella catarrhalisOtitis MediaAcute DiseaseAnti-Bacterial AgentsBacteriaChildHaemophilus influenzaeHumansInfantMultiplex Polymerase Chain ReactionNasopharynxReproducibility of ResultsStreptococcus pneumoniaeAnti-Bacterial AgentsAcute otitis mediaOtopathogensPediatricsPolymerase chain reaction

Identifiers

PMID37549633
PMCPMC10529968
OpenAlexW4385235048

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.