Evidence map›Paper›PMID 37547711›Full record

ArticlePeerJ2023

Salt and osmotic stress can improve the editing efficiency of CRISPR/Cas9-mediated genome editing system in potato.

Mingwang Ye, Mengfan Yao, Canhui Li, Ming Gong

Abstract read
In one paragraph

Article in PeerJ, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Mingwang YeSchool of Life Sciences, Yunnan Normal University, Kunming, Yunnan, China.
Mengfan YaoSchool of Life Sciences, Yunnan Normal University, Kunming, Yunnan, China.
Canhui LiYunnan Key Laboratory of Potato Biology, Yunnan Normal University, Kunming, Yunnan, China.
Ming GongSchool of Life Sciences, Yunnan Normal University, Kunming, Yunnan, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

CRISPR/Cas9-mediated genome editing technology has been widely used for the study of gene function in crops, but the differences between species have led to widely varying genome editing efficiencies. The present study utilized a potato hairy root genetic transformation system and incorporated a rapid assay with GFP as a screening marker. The results clearly demonstrated that salt and osmotic stress induced by NaCl (10 to 50 mM) and mannitol (50 to 200 mM) treatments significantly increased the positive rates of genetic transformation mediated by

Indexed as

Gene EditingSolanum tuberosumCRISPR-Cas SystemsOsmotic PressureSodium ChlorideSodium ChlorideA. rhizogenesCRISPR/Cas9Genome editing efficiencyPotatoSalt and osmotic stress

Identifiers

PMID37547711
PMCPMC10399558

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.