Evidence map›Paper›PMID 37546949›Full record

ArticlebioRxiv : the preprint server for biology2023

Evaluation of gliovascular functions of Aqp4 readthrough isoforms.

Shayna M Mueller, Kelli McFarland White, Stuart B Fass, Siyu Chen, Zhan Shi, Xia Ge, John A Engelbach, Seana H Gaines, Annie R Bice, Michael J Vasek and 6 more

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 2 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

16 authors at 4 institutions in 2 countries.

Shayna M MuellerDepartment of Genetics, Washington University School of Medicine, Saint Louis, MO 63110, USA.
Kelli McFarland WhiteDepartment of Genetics, Washington University School of Medicine, Saint Louis, MO 63110, USA.
Stuart B FassDepartment of Genetics, Washington University School of Medicine, Saint Louis, MO 63110, USA.
Siyu ChenDepartment of Genetics, Washington University School of Medicine, Saint Louis, MO 63110, USA.
Zhan ShiDepartment of Biological Sciences, University of Texas at Dallas, Richardson, Texas 75080, USA.
Xia GeDepartment of Radiology, Washington University School of Medicine, Saint Louis, MO 63110, USA.
John A EngelbachDepartment of Radiology, Washington University School of Medicine, Saint Louis, MO 63110, USA.
Seana H GainesDepartment of Radiology, Washington University School of Medicine, Saint Louis, MO 63110, USA.
Annie R BiceDepartment of Radiology, Washington University School of Medicine, Saint Louis, MO 63110, USA.
Michael J VasekDepartment of Genetics, Washington University School of Medicine, Saint Louis, MO 63110, USA.
Joel R GarbowDepartment of Radiology, Washington University School of Medicine, Saint Louis, MO 63110, USA.
Joseph P CulverDepartment of Radiology, Washington University School of Medicine, Saint Louis, MO 63110, USA.
Zila Martinez-LozadaDepartment of Pediatrics, The Children's Hospital of Philadelphia, Philadelphia, PA 19104, USA.
Martine Cohen-SalmonCenter for Interdisciplinary Research in Biology (CIRB), Collège de France, CNRS, INSERM, Université PSL, Paris, France.
Joseph D DoughertyDepartment of Genetics, Washington University School of Medicine, Saint Louis, MO 63110, USA.
Darshan SapkotaDepartment of Biological Sciences, University of Texas at Dallas, Richardson, Texas 75080, USA.
Washington University in St. Louis · USThe University of Texas at Dallas · USCentre National de la Recherche Scientifique · FRChildren's Hospital of Philadelphia · US

Funding

WUIDDRC Supplement-Supporting the health and well-being of children with intellectual and developmental disability during COVID-19 pandemicP50HD103525 · NICHD · WASHINGTON UNIVERSITY · PI JOSEPH D DOUGHERTY, Natasha Marrus · 2020 to 2026
$15.5M
HIGHLY PARALLEL ANALYSIS OF 5' AND 3' UTR VARIANTS IN NEURODEVELOPMENTAL DISORDERSR01MH116999 · NIMH · WASHINGTON UNIVERSITY · PI JOSEPH D DOUGHERTY · 2018 to 2026
$5.1M
Regulation of local translation in gliaR01NS102272 · NINDS · WASHINGTON UNIVERSITY · PI JOSEPH D DOUGHERTY · 2017 to 2026
$3.8M
Neurobiological significance of Aqp4 stop codon readthroughR00AG061231 · NIA · UNIVERSITY OF TEXAS DALLAS · PI SAPKOTA, DARSHAN · 2021 to 2023
$722k
NIA NIH HHS R00 AG061231NICHD NIH HHS P50 HD103525NIMH NIH HHS R01 MH116999NINDS NIH HHS R01 NS102272
6 · The paper itself

Abstract

Aquaporin-4 (AQP4) is a water channel protein that links astrocytic endfeet to the blood-brain barrier (BBB) and regulates water and potassium homeostasis in the brain, as well as the glymphatic clearance of waste products that would otherwise potentiate neurological diseases. Recently, translational readthrough was shown to generate a C-terminally extended variant of AQP4, known as AQP4x, that preferentially localizes around the BBB through interaction with the scaffolding protein α-syntrophin, and loss of AQP4x disrupts waste clearance from the brain. To investigate the function of AQP4x, we generated a novel mouse AQP4 line (AllX) to increase relative levels of the readthrough variant above the ~15% of AQP4 in the brain of wildtype (WT) mice. We validated the line and assessed characteristics that are affected by the presence of AQP4x, including AQP4 and α-syntrophin localization, integrity of the BBB, and neurovascular coupling. We compared AllX

Identifiers

PMID37546949
PMCPMC10401933
OpenAlexW4385257530

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.