Evidence map›Paper›PMID 37546762›Full record

ArticlebioRxiv : the preprint server for biology2023

Kinetic dissection of pre-crRNA binding and processing by CRISPR-Cas12a.

Selma Sinan, Nathan M Appleby, Rick Russell

Abstract readPreprint
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

3 authors.

Selma SinanDepartment of Molecular Biosciences, University of Texas at Austin, Austin TX 78712.
Nathan M ApplebyDepartment of Molecular Biosciences, University of Texas at Austin, Austin TX 78712.
Rick RussellDepartment of Molecular Biosciences, University of Texas at Austin, Austin TX 78712.

Funding

Mechanisms and Specificity of Proteins that Manipulate DNA and RNA StructureR35GM131777 · NIGMS · UNIVERSITY OF TEXAS AT AUSTIN · PI Rick Russell · 2019 to 2026
$3.2M
NIGMS NIH HHS R35 GM131777
6 · The paper itself

Abstract

CRISPR-Cas12a binds and processes a single pre-crRNA during maturation, providing a simple tool for genome editing applications. Here, we constructed a kinetic and thermodynamic framework for pre-crRNA processing by Cas12a

Indexed as

AsCas12aCRISPR-Cas systemrate-limiting bindingRNA foldingRNA-guided nuclease

Identifiers

PMID37546762
PMCPMC10402064

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.