ArticleSTAR protocols2023
Protocol to perform dynamic microfluidic single-cell cultivation of C. glutamicum.
Article in STAR protocols, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed, 15 citations in OpenAlex.
- Do seconds make a difference? Investigating strain-specific behavior of yeast in dynamic glucose environments.Microbial cell factories · 2026Article
- Physiology and Robustness of Yeasts Exposed to Dynamic pH and Glucose Environments.Biotechnology and bioengineering · 2025Article
- Quantifying microbial robustness in dynamic environments using microfluidic single-cell cultivation.Microbial cell factories · 2024Article
- Four ways of implementing robustness quantification in strain characterisation.Biotechnology for biofuels and bioproducts · 2023Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Here, we present a protocol for the design, fabrication, and usage of a polydimethylsiloxane (PDMS)-based chip for dynamic microfluidic single-cell cultivation of Corynebacterium glutamicum. We describe steps for flow profile establishment and biological preparation. We then detail time-lapse imaging to observe reactions of C. glutamicum to repeated environmental changes in the range of seconds. This system can be adapted to other organisms with a cell wall and soluble non-gaseous environmental factors like nutrients. For complete details on the use and execution of this protocol, please refer to Täuber et al..
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Registered trials
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