Evidence map›Paper›PMID 37542535›Full record

ArticleAnalytical and bioanalytical chemistry2023

Gas-phase stability and thermodynamics of ligand-bound, binary complexes of chloramphenicol acetyltransferase reveal negative cooperativity.

Alexis N Edwards, Anthony J Blue, Jessica M Conforti, Michael S Cordes, Michael A Trakselis, Elyssia S Gallagher

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Analytical and bioanalytical chemistry, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.0field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 7 citations in OpenAlex.

  1. Response Factor Correction for Quantitative Determination of HomooligomericJournal of the American Society for Mass Spectrometry · 2026
    Article
  2. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Alexis N EdwardsDepartment of Chemistry and Biochemistry, Baylor University, Waco, TX, 76798, USA.
Anthony J BlueDepartment of Chemistry and Biochemistry, Baylor University, Waco, TX, 76798, USA.
Jessica M ConfortiDepartment of Chemistry and Biochemistry, Baylor University, Waco, TX, 76798, USA.
Michael S CordesDepartment of Chemistry and Biochemistry, Baylor University, Waco, TX, 76798, USA.
Michael A TrakselisDepartment of Chemistry and Biochemistry, Baylor University, Waco, TX, 76798, USA.ORCID http://orcid.org/0000-0001-7054-8475
Elyssia S GallagherDepartment of Chemistry and Biochemistry, Baylor University, Waco, TX, 76798, USA. elyssia_gallagher@baylor.edu.ORCID http://orcid.org/0000-0002-5411-7285
Baylor University · US

Funding

KDM6A function in epithelial-mesenchymal plasticityR15GM146188 · NIGMS · BAYLOR UNIVERSITY · PI TAUBE, JOSEPH H. · 2022 to 2023
$493k
NIGMS NIH HHS 1R15GM146188-01NIGMS NIH HHS R15 GM146188
6 · The paper itself

Abstract

The biological role of the bacterial chloramphenicol (Chl)-resistance enzyme, chloramphenicol acetyltransferase (CAT), has seen renewed interest due to the resurgent use of Chl against multi-drug-resistant microbes. This looming threat calls for more rationally designed antibiotic derivatives that have improved antimicrobial properties and reduced toxicity in humans. Herein, we utilize native ion mobility spectrometry-mass spectrometry (IMS-MS) to investigate the gas-phase structure and thermodynamic stability of the type I variant of CAT from Escherichia coli (EcCAT

Indexed as

Escherichia coliAcetyl Coenzyme AChloramphenicol O-AcetyltransferaseHumansLigandsMass SpectrometryThermodynamicsAcetyl Coenzyme AChloramphenicol O-AcetyltransferaseLigandsChloramphenicol acetyltransferase (CAT)Collision-induced dissociation (CID)Collision-induced unfolding (CIU)Ligand bindingMass spectrometry (MS)Protein stability

Identifiers

PMID37542535
PMCPMC12306972
OpenAlexW4385607086

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.