ArticleThe Plant cell2023
The transcription factors ZmNAC128 and ZmNAC130 coordinate with Opaque2 to promote endosperm filling in maize.
Article in The Plant cell, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 61 citations in OpenAlex.
- ZmSK1 encodes a sucrose synthase essential for starch synthesis and endosperm development in maize.Plant molecular biology · 2026Article
- A plastoglobuli-localized enzyme links phenylalanine biosynthesis to translational homeostasis in maize.Nature communications · 2026Article
- Integrated physiological and transcriptomic measurements reveal changes in the accumulation and partitioning of storage reserves after prevention of pollination in maize.BMC plant biology · 2026Article
- Transcriptional networks shaping malting quality in barley: From grain development to brewing performance.Food chemistry. Molecular sciences · 2026Review
- The ZmCOP1s-ZmCOL3 Module Enhances Late Flowering, Grain Yield and Grain Quality in Maize.Plant biotechnology journal · 2026Article
- Discovering of the transcriptional regulatory network involved in starch biosynthesis in sorghum grains.Plant diversity · 2026Article
- ZmbZIP39: orchestrating source, flow, and sink for maize grain development.The Plant cell · 2026Article
- Cereal protein biofortification at the interface of nutrition, yield and sustainability.Nature plants · 2026Review
- Subclass III SnRK2 Kinases Coordinate Starch and Storage Protein Synthesis During Maize Kernel Development.Plant biotechnology journal · 2026Article
- Meta-QTLs and candidate genes for kernel protein content in maize.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- ZmMYB127 controls maize endosperm filling via dual-transcriptional regulation to improve grain yield and quality.Nature plants · 2026Article
- ZmMYB127 Modulates Maize Kernel Texture and Size by Integrating the Synthesis of Starch, Zein Proteins and Auxin.Plant biotechnology journal · 2026Article
- Transactivation of AGPL2 by Opaque2 is responsible for starch accumulation and kernel hardness in sweet corn.Journal of experimental botany · 2026Article
- From development to yield: genetic and molecular regulation of agronomic traits in maize seeds.Frontiers in plant science · 2026Review
- Transcriptome and Metabolome Analyses Reveal the Accumulation Mechanism of Carbohydrates DuringBiomolecules · 2025Article
- The bHLH transcription factor PIL1 orchestrates starch synthesis in key cereal crops.Nature communications · 2025Article
- Decades' progress and prospects on maize functional genomics and molecular breeding.Science China. Life sciences · 2025Review
- Dual-Layer Transcriptional-Protein Regulation by HvAP2-12 Represses HvAP2-18 Activity to Fine-Tune Barley Starch Synthesis.Plant biotechnology journal · 2025Article
- Genome-wide identification and functional analysis of the polyamine metabolism-related genes in Foxtail millet (Setaria Italica L.).Functional & integrative genomics · 2025Article
- Regulatory Mechanisms of Free Umami Amino Acid Accumulation in Fresh Waxy Kernels: Insights from Transcriptome and Metabolomics Analyses.Foods (Basel, Switzerland) · 2025Article
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Authors and funding
15 authors at 2 institutions in 1 country.
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Abstract
Endosperm filling in maize (Zea mays), which involves nutrient uptake and biosynthesis of storage reserves, largely determines grain yield and quality. However, much remains unclear about the synchronization of these processes. Here, we comprehensively investigated the functions of duplicate NAM, ATAF1/2, and CUC2 (NAC)-type transcription factors, namely, ZmNAC128 and ZmNAC130, in endosperm filling. The gene-edited double mutant zmnac128 zmnac130 exhibits a poorly filled kernel phenotype such that the kernels have an inner cavity. RNA sequencing and protein abundance analysis revealed that the expression of many genes involved in the biosynthesis of zein and starch is reduced in the filling endosperm of zmnac128 zmnac130. Further, DNA affinity purification and sequencing combined with chromatin-immunoprecipitation quantitative PCR and promoter transactivation assays demonstrated that ZmNAC128 and ZmNAC130 are direct regulators of 3 (16-, 27-, and 50-kD) γ-zein genes and 6 important starch metabolism genes (Brittle2 [Bt2], pullulanase-type starch debranching enzyme [Zpu1], granule-bound starch synthase 1 [GBSS1], starch synthase 1 [SS1], starch synthase IIa [SSIIa], and sucrose synthase 1 [Sus1]). ZmNAC128 and ZmNAC130 recognize an additional cis-element in the Opaque2 (O2) promoter to regulate its expression. The triple mutant zmnac128 zmnac130 o2 exhibits extremely poor endosperm filling, which results in more than 70% of kernel weight loss. ZmNAC128 and ZmNAC130 regulate the expression of the transporter genes sugars that will eventually be exported transporter 4c (ZmSWEET4c), sucrose and glucose carrier 1 (ZmSUGCAR1), and yellow stripe-like2 (ZmYSL2) and in turn facilitate nutrient uptake, while O2 plays a supporting role. In conclusion, ZmNAC128 and ZmNAC130 cooperate with O2 to facilitate endosperm filling, which involves nutrient uptake in the basal endosperm transfer layer (BETL) and the synthesis of zeins and starch in the starchy endosperm (SE).
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