ArticleJournal of advanced research2024
Resuscitation of viable but nonculturable bacteria promoted by ATP-mediated NAD
Article in Journal of advanced research, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
10 citing papers in PubMed, 30 citations in OpenAlex.
- Effects of Nutritional Conditions and Exogenous N-Acyl-Homoserine Lactones on the Resuscitation of Sodium Hypochlorite-Induced DormantMicroorganisms · 2026Article
- Growth phase-specific induction of a viable but nonculturable (VBNC) state inMicrobiology spectrum · 2026Article
- Preliminary study of the growth variability ofFrontiers in microbiology · 2026Article
- The Effect of Non-Thermal Processing on the Fate of Pathogenic Bacteria and Hidden Hazardous Risks.Foods (Basel, Switzerland) · 2025Review
- The Mla pathway promotesmBio · 2025Article
- Drug-induced differential culturability in diverse strains of Mycobacterium tuberculosis.Scientific reports · 2025Article
- Investigating the Antibacterial Effect of a Novel Gallic Acid-Based Green Sanitizer Formulation.Foods (Basel, Switzerland) · 2024Article
- The viable but non-culturable (VBNC) status of Shewanella putrefaciens (S. putrefaciens) with thermosonication (TS) treatment.Ultrasonics sonochemistry · 2024Article
- Preliminary Study of the Characterization of the Viable but Noncultivable State ofMicroorganisms · 2024Article
- The Viable but Non-Culturable (VBNC) State, a Poorly Explored Aspect of Beneficial Bacteria.Microorganisms · 2023Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionEntry into the viable but nonculturable (VBNC) state is a survival strategy adopted by bacteria to survive harsh environment. Although VBNC cells still have metabolic activity, they lose the ability to form colonies on nonselective culture media. Thus, conventional bacterial detection methods, such as plate counting, are unable to detect the presence of VBNC cells. When the environmental conditions are appropriate, VBNC cells can initiate resuscitation, posing a great risk to the safety of public health. The study of the VBNC resuscitation mechanism could provide new insights into the prevention and control of VBNC resuscitation.
objectivesUncovering the molecular mechanism of VBNC cell resuscitation by investigating the role of O-antigen ligase (RfaL) in inhibiting the resuscitation of Escherichia coli O157:H7 in the VBNC state.
methodsRfaL was screened and verified as a resuscitation inhibitor of VBNC Escherichia coli O157:H7 by detecting resuscitation curve and time-lapse microscopy. The mechanism of RfaL impacts VBNC E. coli resuscitation was investigated by detecting the single cell ATP content, metabolomic changes, NAD(H) content and new protein biosynthesis of WT and ΔrfaL at different stage of resuscitation.
resultsMutation of rfaL, which encoded an O-antigen ligase, markedly shortened the resuscitating lag phase. Further studies indicated that ΔrfaL VBNC cells contained higher ATP levels, and ATP consumption during the resuscitating lag phase was highly correlated with resuscitation efficiency. Metabolomic analysis revealed that ATP was utilized to activate the Handler and salvage pathways to synthesize NAD
conclusionOur findings revealed a strategy employed by VBNC cells for revival, that is, using residual ATP to primarily recover metabolic activity, driving cells to exit dormancy. The synthesis pathway of lipopolysaccharide (LPS) in rfaL null mutant was inhibited and could supply more ATP to synthesis NAD
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