Evidence map›Paper›PMID 37540370›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Expression and Functional Studies of Leaf, Floral, and Fruit Developmental Genes in Non-model Species.

Natalia Pabón-Mora, Harold Suárez-Baron, Yesenia Madrigal, Juan F Alzate, Favio González

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Natalia Pabón-MoraInstituto de Biología, Universidad de Antioquia, Medellín, Colombia. lucia.pabon@udea.edu.co.
Harold Suárez-BaronInstituto de Biología, Universidad de Antioquia, Medellín, Colombia.
Yesenia MadrigalInstituto de Biología, Universidad de Antioquia, Medellín, Colombia.
Juan F AlzateCentro Nacional de Secuenciación Genómica-CNSG, Sede de Investigación Universitaria-SIU, Medellín, Antioquia, Colombia.
Favio GonzálezInstituto de Ciencias Naturales, Universidad Nacional de Colombia, Sede Bogotá, Bogotá, Colombia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Researchers working on evolutionary developmental plant biology are inclined to choose non-model taxa to address how specific features have been acquired during ontogeny and fixed during phylogeny. In this chapter we describe methods to extract RNA, to assemble de-novo transcriptomes, to isolate orthologous genes within gene families, and to evaluate expression and function of target genes. We have successfully optimized these protocols for non-model plant species including ferns, gymnosperms, and a large assortment of angiosperms. In the latter, we have ranged a large number of families including Aristolochiaceae, Apodanthaceae, Chloranthaceae, Orchidaceae, Papaveraceae, Rubiaceae, Solanaceae, and Tropaeolaceae.

Indexed as

FernsFruitEvolution, MolecularGene Expression Regulation, PlantGenes, DevelopmentalPhylogenyPlant LeavesPlant ProteinsPlantsPlant ProteinsExpression analysesFunctional analysesNon-model plantsOrthologous genes identificationTranscriptomics

Identifiers

PMID37540370

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.