ArticleMethods in molecular biology (Clifton, N.J.)2023
RNA In Situ Hybridization on Plant Tissue Sections: Expression Analysis at Cellular Resolution.
Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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Who cites it
7 citing papers in PubMed, 7 citations in OpenAlex.
- Dodder seedlings benefit from apoplastic nutrition by inducing SWEET15-mediated unloading of sucrose from the host's phloem.The New phytologist · 2026Article
- Laminar patterning transcription factors orchestrate spatial metabolite partitioning in Capsicum fruit.Nature plants · 2026Article
- Visualizing application of RUBY-MAT system in maize genetic transformation and gene expression pattern detection.Plant cell reports · 2026Article
- The trehalose 6-phosphate pathway coordinates dynamic changes at the shoot apical meristem in Arabidopsis thaliana.Plant physiology · 2025Article
- GERMIN3 regulates tuber initiation and axillary bud activation by facilitating plasmodesmatal gating.The Plant journal : for cell and molecular biology · 2025Article
- Carbon and nitrogen signaling regulate FLOWERING LOCUS C and impact flowering time in Arabidopsis.Plant physiology · 2024Article
- A GDSL-motif Esterase/Lipase Affects Wax and Cutin Deposition and Controls Hull-Caryopsis Attachment in Barley.Plant & cell physiology · 2024Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA in situ hybridization offers a means to study the spatial expression of candidate genes by making use of specific, labelled RNA probes on thin tissue sections. Unlike other methods, such as promoter GUS fusions, for which all regulatory sequences should be available and transgenic plants have to be generated, RNA in situ hybridization allows specific and direct detection of even low abundant transcripts at cellular resolution. Although various protocols exist, the results published throughout the literature indicate a very obvious problem of the technique: each step has the potential to affect the outcome, that is, the signal strength, presence or absence of background, and visibility of individual cells. The protocol described here tries to avoid all these problems by addressing each step in detail and providing advice regarding critical steps for a distinct visualization of gene expression on intact tissue sections without any background.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.