Evidence map›Paper›PMID 37536273›Full record

ArticleInflammatory bowel diseases2024

Exclusive Enteral Nutrition Alleviates Th17-Mediated Inflammation via Eliminating Mechanical Stress-Induced Th17-Polarizing Cytokines in Crohn's-like Colitis.

Ramasatyaveni Geesala, Ke Zhang, You-Min Lin, John C Johnson, Yingzi Cong, Steven Cohn, Xuan-Zheng Shi

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Inflammatory bowel diseases, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
2.1field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 10 citations in OpenAlex.

  1. Article
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  3. Mechanical stretch promotes sustained proliferation and inflammation in developing human airway smooth muscle.American journal of physiology. Lung cellular and molecular physiology · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Ramasatyaveni GeesalaDepartment of Internal Medicine, University of Texas Medical Branch at Galveston, Galveston, TX, USA.
Ke ZhangDepartment of Internal Medicine, University of Texas Medical Branch at Galveston, Galveston, TX, USA.
You-Min LinDepartment of Internal Medicine, University of Texas Medical Branch at Galveston, Galveston, TX, USA.
John C JohnsonDepartment of Internal Medicine, University of Texas Medical Branch at Galveston, Galveston, TX, USA.
Yingzi CongDepartment of Microbiology and Immunology, University of Texas Medical Branch at Galveston, Galveston, TX, USA.ORCID 0000-0003-4167-7395
Steven CohnDepartment of Internal Medicine, University of Texas Medical Branch at Galveston, Galveston, TX, USA.
Xuan-Zheng ShiDepartment of Internal Medicine, University of Texas Medical Branch at Galveston, Galveston, TX, USA.ORCID 0000-0002-9014-853X
The University of Texas Medical Branch at Galveston · US

Funding

Pathogenic Role of Mechanical Stress in Fibrosis and Tissue Remodeling in Crohn's DiseaseR01DK124611 · NIDDK · UNIVERSITY OF TEXAS MED BR GALVESTON · PI SHI, XUAN-ZHENG PETER · 2020 to 2023
$1.4M
NIDDK NIH HHS R01 DK124611NIH HHS R01 DK124611
6 · The paper itself

Abstract

BACKGROUND AND

aimsExclusive enteral nutrition (EEN) with a liquid diet is the only established dietary treatment for Crohn's' disease (CD). However, the mechanism of action of EEN in CD is unclear. T helper 17 (Th17) immune response plays a critical role in CD. We hypothesized that EEN alleviates Th17 response by eliminating mechanical stress-induced expression of Th17-polarizing cytokines.

methodsA rat model of Crohn's-like colitis was established by intracolonic instillation of TNBS (65 mg/kg in 250 µL of 40% ethanol). Control rats were treated with saline. We characterized immunophenotypes and molecular changes of the colon in control and colitis rats with and without EEN treatment. Th17 differentiation was determined using coculture assays.

resultsTNBS instillation induced transmural inflammation with stenosis in the inflammation site and a marked increase of Th17-polarizing cytokines interleukin (IL)-6 and osteopontin and the Th17 cell population in the mechanically distended preinflammation site (P-site). EEN treatment eliminated mechanical distention and the increase of IL-6, osteopontin, and Th17 response in the P-site. IL-6 and osteopontin expression was found mainly in the muscularis externa. Mechanical stretch of colonic smooth muscle cells in vitro induced a robust increase of IL-6 and osteopontin. When naïve T cells were cultured with conditioned media from the P-site tissue or stretched cells, Th17 differentiation was significantly increased. Inhibition of IL-6, but not deletion of osteopontin, blocked the increase of Th17 differentiation.

conclusionsMechanical stress induces Th17-polarizing cytokines in the colon. EEN attenuates Th17 immune response by eliminating mechanical stress-induced IL-6 in Crohn's-like colitis.

Indexed as

ColitisCrohn DiseaseAnimalsCytokinesEnteral NutritionInflammationInterleukin-6OsteopontinRatsStress, MechanicalCytokinesInterleukin-6OsteopontinCrohn’s diseaseexclusive enteral nutritionIL-6mechanical stressTh17 immune response

Identifiers

PMID37536273
PMCPMC10906353
OpenAlexW4385516320

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.