ArticleFrontiers in cellular and infection microbiology2023
High throughput Luminex beads based multiplex assay for identification of six major bacterial pathogens of mastitis in dairy animals.
Article in Frontiers in cellular and infection microbiology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 3 citations in OpenAlex.
- A Multiplex Liquid Array Assay Based on Luminex xTAG Technology for the Detection of Five Respiratory Pathogens in SPF Chickens.Veterinary sciences · 2026Article
- Development and Application of a Dual-Readout RPA-PfAgo System for Rapid Detection ofVeterinary sciences · 2026Article
- Differences in Peripheral Blood Gene Expression of Xinjiang Brown Cattle with Varying Somatic Cell Counts.Biology · 2026Article
- Novel multiplex Magpix assay platforms for simultaneous detection of different serotypes of BTV circulating in India.Virus genes · 2026Article
- Serological evidence uncovers undocumented mpox exposure in febrile patients in Nigeria.Frontiers in public health · 2026Article
- Development of a multiplexed Luminex assay for simultaneous detection of enteric viruses in cattle.Veterinary research forum : an international quarterly journal · 2024Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
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Abstract
Introduction: Bovine mastitis is caused by over 150 different microorganisms. Specific identification and quantification of multiple bacteria in a single milk sample becomes essential for rapid intervention. Methods: In the present study a Luminex beads based multiplex assay emphasizing on the precise identification of six major bacterial pathogens of mastitis was developed. Assay was developed in two triplex sets, triplex 1 comprised of Results: The analytical sensitivity was 10 6 copies per reaction mixture for all the six bacteria. A 100% analytical specificity was observed for simultaneous detection of these bacteria. Clinical milk samples from 100 bovine quarters were tested for validation. Discussion: The analytical sensitivity was similar to the findings reported earlier in real time PCR multiplex assay targeting the DNA of the 11 most common bacterial species or groups in mastitis. The analytical specificity of the optimized assay was 100% similar to reported earlier for simultaneous detection of Mycoplasma spp. and for seven entric viruses of humans.The developed assay indicates a concept proof of a rapid, cost effective high throughput diagnostic tool for identification of major bacteria causing mastitis.
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