ArticleComputational and structural biotechnology journal2023
Nanopore sequencing of PCR products enables multicopy gene family reconstruction.
Article in Computational and structural biotechnology journal, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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7 citing papers in PubMed, 10 citations in OpenAlex.
- Genomic islands in Wolbachia prophages drive amplification and diversification of cytoplasmic incompatibility genes in Culex pipiens.Genetics · 2026Article
- Extensive mobilome dynamics in a widespread endosymbiont: long read metagenomics reveal dimeric plasmids and highly fragmented prophages in Wolbachia from Culex pipiens.Research square · 2026Article
- Long-range PCR and Nanopore sequencing for localisation and phasing variants: an end-to-end clinical application workflow.BMC medical genomics · 2025Article
- Recombination, Truncation and Horizontal Transfer Shape the Diversity of Wolbachia-induced Cytoplasmic Incompatibility Patterns.Molecular biology and evolution · 2025Article
- The genetic puzzle of multicopy genes: challenges and troubleshooting.Plant methods · 2025Article
- Despite structural identity, ace-1 heterogenous duplication resistance alleles are quite diverse in Anopheles mosquitoes.Heredity · 2024Article
- Intra-lineage microevolution of Wolbachia leads to the emergence of new cytoplasmic incompatibility patterns.PLoS biology · 2024Article
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The importance of gene amplifications in evolution is more and more recognized. Yet, tools to study multi-copy gene families are still scarce, and many such families are overlooked using common sequencing methods. Haplotype reconstruction is even harder for polymorphic multi-copy gene families. Here, we show that all variants (or haplotypes) of a multi-copy gene family present in a single genome, can be obtained using Oxford Nanopore Technologies sequencing of PCR products, followed by steps of mapping, SNP calling and haplotyping. As a proof of concept, we acquired the sequences of highly similar variants of the
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