Evidence map›Paper›PMID 37533651›Full record

ArticleCell reports methods2023

Targeted insertion and reporter transgene activity at a gene safe harbor of the human blood fluke,

Wannaporn Ittiprasert, Max F Moescheid, Cristian Chaparro, Victoria H Mann, Thomas Quack, Rutchanee Rodpai, André Miller, Prapakorn Wisitpongpun, Watunyoo Buakaew, Margaret Mentink-Kane and 5 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports methods, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
2.8field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 10 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Review
  5. Article
  6. Review
  7. Review
  8. Trematode Genomics and Proteomics.Advances in experimental medicine and biology · 2024
    Article
  9. Praziquantel activates a native cation current inFrontiers in parasitology · 2023
    Article
  10. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 5 institutions in 4 countries.

Wannaporn IttiprasertDepartment of Microbiology, Immunology & Tropical Medicine, School of Medicine & Health Sciences, George Washington University, Washington, DC 20037, USA.
Max F MoescheidDepartment of Microbiology, Immunology & Tropical Medicine, School of Medicine & Health Sciences, George Washington University, Washington, DC 20037, USA.
Cristian ChaparroIHPE, University of Perpignan Via Domitia, CNRS, IFREMER, University Montpellier, Perpignan, France.
Victoria H MannDepartment of Microbiology, Immunology & Tropical Medicine, School of Medicine & Health Sciences, George Washington University, Washington, DC 20037, USA.
Thomas QuackInstitute of Parasitology, Biomedical Research Center Seltersberg, Justus Liebig University Giessen, Giessen, Germany.
Rutchanee RodpaiDepartment of Microbiology, Immunology & Tropical Medicine, School of Medicine & Health Sciences, George Washington University, Washington, DC 20037, USA.
André MillerSchistosomiasis Resource Center, Biomedical Research Institute, Rockville, MD 20850, USA.
Prapakorn WisitpongpunDepartment of Microbiology, Immunology & Tropical Medicine, School of Medicine & Health Sciences, George Washington University, Washington, DC 20037, USA.
Watunyoo BuakaewDepartment of Microbiology, Immunology & Tropical Medicine, School of Medicine & Health Sciences, George Washington University, Washington, DC 20037, USA.
Margaret Mentink-KaneSchistosomiasis Resource Center, Biomedical Research Institute, Rockville, MD 20850, USA.
Sarah SchmidSchistosomiasis Resource Center, Biomedical Research Institute, Rockville, MD 20850, USA.
Anastas PopratiloffNanofabrication and Imaging Center, Science & Engineering Hall, George Washington University, Washington, DC 20052, USA.
Christoph G GreveldingInstitute of Parasitology, Biomedical Research Center Seltersberg, Justus Liebig University Giessen, Giessen, Germany.
Christoph GrunauIHPE, University of Perpignan Via Domitia, CNRS, IFREMER, University Montpellier, Perpignan, France.
Paul J BrindleyDepartment of Microbiology, Immunology & Tropical Medicine, School of Medicine & Health Sciences, George Washington University, Washington, DC 20037, USA.
George Washington University · USBiomedical Research Institute · USCentre National de la Recherche Scientifique · FRJustus-Liebig-Universität Gießen · DEKhon Kaen University · TH

Funding

Targeting parasite-host communication to combat liver fluke-induced bile duct cancerR01CA164719 · NCI · GEORGE WASHINGTON UNIVERSITY · PI Paul J Brindley, THEWARACH LAHA · 2012 to 2026
$5.0M
Acquisition of Zeiss 710 Confocal Laser Scanning MicroscopeS10RR025565 · NCRR · GEORGE WASHINGTON UNIVERSITY · PI POPRATILOFF, ANASTAS · 2009 to 2009
$500k
NCI NIH HHS R01 CA164719NCRR NIH HHS S10 RR025565Wellcome Trust
6 · The paper itself

Abstract

The identification and characterization of genomic safe harbor sites (GSHs) can facilitate consistent transgene activity with minimal disruption to the host cell genome. We combined computational genome annotation and chromatin structure analysis to predict the location of four GSHs in the human blood fluke,

Indexed as

Gene EditingSchistosoma mansoniAnimalsAnimals, Genetically ModifiedHumansTransgenesgene safe harborhuman blood flukeoverlapping CRISPR targetreporter transgeneSchistosoma mansoni

Identifiers

PMID37533651
PMCPMC10391569
OpenAlexW4385197987

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.