ArticleCell reports methods2023
A structured evaluation of cryopreservation in generating single-cell transcriptomes from cerebrospinal fluid.
Article in Cell reports methods, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- A CSF disease-associated macrophage signature defines progressive multiple sclerosis.Journal of neuroinflammation · 2026Article
- The cerebrospinal fluid immune microenvironment in lung cancer leptomeningeal metastasis: cellular ecosystems and therapeutic possibilities.Frontiers in immunology · 2026Review
- Multisite Assessment of Methods for Cell Preservation Upstream of Single-Cell RNA Sequencing.Journal of biomolecular techniques : JBT · 2026Article
- Single-cell profiling of blood and cerebrospinal fluid in tuberculous meningitis.Journal of immunology (Baltimore, Md. : 1950) · 2025Article
- Multi-site Assessment of Methods for Cell Preservation Upstream of Single Cell RNA Sequencing.bioRxiv : the preprint server for biology · 2025Article
- Detection of Human Brain Cancers using Genomic and Immune Cell Characterization of Cerebrospinal Fluid through CSF-BAM.Cancer discovery · 2025Article
- The CXCL16/CXCR6 axis is linked to immune effector cell-associated neurotoxicity in chimeric antigen receptor (CAR) T cell therapy.Genome medicine · 2025Article
- RORγt-expressing dendritic cells are functionally versatile and evolutionarily conserved antigen-presenting cells.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Single-cell transcriptomics for immune profiling of cerebrospinal fluid in neurological diseases.Frontiers in immunology · 2025Review
- Enhanced cell survival in prepubertal testicular tissue cryopreserved with membrane lipids and antioxidants rich cryopreservation medium.Cell and tissue research · 2025Article
- Chemokine-mediated cell migration into the central nervous system in progressive multifocal leukoencephalopathy.Cell reports. Medicine · 2024Article
- CHIT1 at diagnosis predicts faster disability progression and reflects early microglial activation in multiple sclerosis.Nature communications · 2024Article
- Cryopreservation of cerebrospinal fluid cells preserves the transcriptional landscape for single-cell analysis.Journal of neuroinflammation · 2024Article
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Authors and funding
16 authors.
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Abstract
Single-cell transcriptomics allows characterization of cerebrospinal fluid (CSF) cells at an unprecedented level. Here, we report a robust cryopreservation protocol adapted for the characterization of fragile CSF cells by single-cell RNA sequencing (RNA-seq) in moderate- to large-scale studies. Fresh CSF was collected from twenty-one participants at two independent sites. Each CSF sample was split into two fractions: one was processed fresh, while the second was cryopreserved for months and profiled after thawing. B and T cell receptor sequencing was also performed. Our comparison of fresh and cryopreserved data from the same individuals demonstrates highly efficient recovery of all known CSF cell types. We find no significant difference in cell type proportions and cellular transcriptomes between fresh and cryopreserved cells. Results were comparable at both sites and with different single-cell sequencing chemistries. Cryopreservation did not affect recovery of T and B cell clonotype diversity. Our CSF cell cryopreservation protocol provides an important alternative to fresh processing of fragile CSF cells.
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