ArticleParasites & vectors2023
Comparison of Mini-FLOTAC, Flukefinder and sedimentation techniques for detection and quantification of Fasciola hepatica and Calicophoron daubneyi eggs using spiked and naturally infected bovine faecal samples.
Article in Parasites & vectors, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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11 citing papers in PubMed, 20 citations in OpenAlex.
- Prevalence of Fasciolosis and Dicrocoeliosis in Slaughtered Cattle and Sheep in Siirt Province, Türkiye: A Comparison of Coprological and Postmortem Findings.Veterinary sciences · 2026Article
- Factors affecting detection and quantification of Schistosoma haematobium eggs in pooled urine samples.PLoS neglected tropical diseases · 2026Article
- A Rapid Visual Detection Method forAnimals : an open access journal from MDPI · 2026Article
- Comparison of wet mount, Mini-FLOTAC and Midi Parasep® coprological techniques for the detection ofInternational journal for parasitology. Parasites and wildlife · 2026Article
- One Health assessment of fascioliasis and schistosomiasis in humans, livestock, and snail vectors in peri-urban Rwanda.Veterinary world · 2026Article
- Fasciolosis: Analytical approaches to post-treatment liver enzyme activity changes in cattle.Helminthologia · 2026Article
- New insights into trematode infections in cattle and their snail intermediate hosts in a Mediterranean area of Italy.Food and waterborne parasitology · 2026Article
- Advancement in Diagnosis, Treatment, and Vaccines againstPathogens (Basel, Switzerland) · 2024Review
- Evaluating the Performance of FlukeCatcher at Detecting Urogenital Schistosomiasis.Diagnostics (Basel, Switzerland) · 2024Article
- Epidemiology of rumen fluke infection in selected buffalo farms in perak, malaysia: prevalence, molecular species identification, and associated risk factors.Parasitology research · 2024Article
- Fasciolosis in ruminants in Brazil.Brazilian journal of veterinary medicine · 2024Review
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Authors and funding
7 authors at 2 institutions in 2 countries.
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Abstract
backgroundFasciolosis (Fasciola hepatica) and paramphistomosis (Calicophoron daubneyi) are two important infections of livestock. Calicophoron daubneyi is the predominant Paramphistomidae species in Europe, and its prevalence has increased in the last 10-15 years. In Italy, evidence suggests that the prevalence of F. hepatica in ruminants is low in the southern part, but C. daubneyi has been recently reported at high prevalence in the same area. Given the importance of reliable tools for liver and rumen fluke diagnosis in ruminants, this study evaluated the diagnostic performance of the Mini-FLOTAC (MF), Flukefinder
methodsBriefly, negative bovine faecal samples were artificially spiked with either F. hepatica or C. daubneyi eggs to achieve different egg count levels: 10, 50 and 100 eggs per gram (EPG) of faeces. Moreover, ten naturally infected cattle farms from southern Italy with either F. hepatica and/or C. daubneyi were selected. For each farm, the samples were analysed individually only with MF technique and as pools using MF, FF and SED techniques. Bayesian latent class analysis (LCA) was used to estimate sensitivity and accuracy of the predicted intensity of infection as well as the infection rate in the naturally infected farms.
resultsThe outcome of this study showed that the highest number of eggs (F. hepatica and C. daubneyi) recovered was obtained with MF, followed by FF and SED in spiked infected samples at 50 and 100 EPG, while at lower infection levels of 10 EPG, FF gave the best results. Moreover, the sensitivity for all the techniques included in the study was estimated at > 90% at infection levels > 20 EPG for both F. hepatica and C. daubneyi eggs. However, MF was the most accurate of the three techniques evaluated to estimate fluke infection intensity. Nevertheless, all three techniques can potentially estimate infection rate at farm level accurately.
conclusionsOptimization and standardization of techniques are needed to improve the FEC of fluke eggs.
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