Evidence map›Paper›PMID 37531174›Full record

ArticleChemical communications (Cambridge, England)2023

Extraction-free, one-pot CRISPR/Cas12a detection of microRNAs directly from extracellular vesicles.

He Yan, Song Han, Steven Hughes, Yong Zeng

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Chemical communications (Cambridge, England), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

He YanDepartment of Chemistry, University of Florida, Gainesville, FL 32611, USA. zengy@ufl.edu.
Song HanDepartment of Surgery, University of Florida College of Medicine, Gainesville, FL 32610, USA.
Steven HughesDepartment of Surgery, University of Florida College of Medicine, Gainesville, FL 32610, USA.
Yong ZengDepartment of Chemistry, University of Florida, Gainesville, FL 32611, USA. zengy@ufl.edu.ORCID http://orcid.org/0000-0003-0537-109X

Funding

Using Integrated Omics to Identify Dysfunctional Genetic Mechanisms Influencing Schizophrenia and Sleep DisturbancesP20GM130423 · NIGMS · UNIVERSITY OF KANSAS MEDICAL CENTER · PI Diane E Mahoney · 2019 to 2026
$21.5M
Extracellular Vesicle Proteomic Fingerprinting of Ovarian Cancer for Early Detection with a Nanoengineered MicrosystemR01CA260132 · NCI · UNIVERSITY OF KANSAS MEDICAL CENTER · PI GODWIN, ANDREW K., ZENG, YONG · 2021 to 2025
$3.4M
Nano-Engineered Lab-on-a-Chip for Assessing HuR-Regulated Exosomes for Cancer Monitoring and Targeted TherapyR01CA243445 · NCI · UNIVERSITY OF KANSAS LAWRENCE · PI DIXON, DAN ALAN, XU, LIANG · 2019 to 2023
$3.0M
Integrated exosomes profiling for minimally invasive diagnosis and monitoring of cancerR33CA214333 · NCI · UNIVERSITY OF KANSAS LAWRENCE · PI GODWIN, ANDREW K., ZENG, YONG · 2017 to 2019
$1.2M
Integrative Functional Profiling of Tumor-Derived Extracellular VesiclesR33CA252158 · NCI · UNIVERSITY OF FLORIDA · PI XU, LIANG, ZENG, YONG · 2021 to 2024
$1.0M
NCI NIH HHS R01 CA243445NCI NIH HHS R01 CA260132NCI NIH HHS R33 CA214333NCI NIH HHS R33 CA252158NIGMS NIH HHS P20 GM130423
6 · The paper itself

Abstract

Current methods for extracellular vesicle (EV) miRNA analysis mostly require RNA extraction, which results in a multi-step, time-consuming workflow. This study reports an extraction-free method that combines thermolysis treatment of EVs with a one-pot EXTRA-CRISPR assay, enabling the vastly simplified analysis of EV miRNAs with a comparable performance to that of the extraction-based assays.

Indexed as

BiochemistryExtracellular VesiclesMicroRNAsBacterial ProteinsCarcinoma, Pancreatic DuctalCell LineCRISPR-Associated ProteinsCRISPR-Cas SystemsEndodeoxyribonucleasesHumansPancreatic NeoplasmsBacterial ProteinsCas12a proteinCRISPR-Associated ProteinsEndodeoxyribonucleasesMicroRNAs

Identifiers

PMID37531174
PMCPMC10478011

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.