Evidence map›Paper›PMID 37531006›Full record

ArticleBrazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]2023

Limosilactobacillus reuteri as sustainable biological control agent against toxigenic Fusarium verticillioides.

Melissa Tiemi Hirozawa, Mario Augusto Ono, Igor Massahiro de Souza Suguiura, Sandra Garcia, Jaqueline Gozzi Bordini, Ismael Rodrigues Amador, Elisa Yoko Hirooka, Elisabete Yurie Sataque Ono

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
3.2field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
  2. The Role of ProbioticsInternational journal of molecular sciences · 2026
    Review
  3. Review
  4. Article
  5. Article
  6. Frontiers in microbiology · 2023
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Melissa Tiemi HirozawaState University of Londrina, Department of Biochemistry and Biotechnology, P.O. Box 10, 011, 86057-970, Londrina, Paraná, Brazil.
Mario Augusto OnoState University of Londrina, Department of Pathological Sciences, P.O. Box 10, 011, 86057-970, Londrina, Paraná, Brazil.
Igor Massahiro de Souza SuguiuraState University of Londrina, Department of Pathological Sciences, P.O. Box 10, 011, 86057-970, Londrina, Paraná, Brazil.
Sandra GarciaState University of Londrina, Department of Food Science and Technology, P.O. Box 10, 011, 86057-970, Londrina, Paraná, Brazil.
Jaqueline Gozzi BordiniState University of Londrina, Department of Biochemistry and Biotechnology, P.O. Box 10, 011, 86057-970, Londrina, Paraná, Brazil.
Ismael Rodrigues AmadorState University of Londrina, Department of Biochemistry and Biotechnology, P.O. Box 10, 011, 86057-970, Londrina, Paraná, Brazil.
Elisa Yoko HirookaState University of Londrina, Department of Food Science and Technology, P.O. Box 10, 011, 86057-970, Londrina, Paraná, Brazil.
Elisabete Yurie Sataque OnoState University of Londrina, Department of Biochemistry and Biotechnology, P.O. Box 10, 011, 86057-970, Londrina, Paraná, Brazil. eysono@uel.br.ORCID http://orcid.org/0000-0003-4461-4525
Universidade Estadual de Londrina · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Corn contamination with Fusarium verticillioides (Sacc.) Nirenberg is a worldwide problem that affects yield and grain quality resulting in severe economic losses and implications for food safety. Control of F. verticillioides is a challenge, but lactic acid bacteria (LAB) has high potential as a biological control agent. In this study, the antifungal effect of Limosilactobacillus reuteri (formerly Lactobacillus reuteri) LR-92 against F. verticillioides 97L was investigated. Cell-free supernatant (CFS) from L. reuteri showed concentration-dependent fungicidal and fungistatic activity against F. verticillioides 97L. The antifungal compounds from CFS showed heat stability and pH dependence, and antifungal activity was not affected by treatment with proteolytic enzymes. High-performance liquid chromatography analysis indicated that L. reuteri LR-92 produces lactic and acetic acids. After liquid-liquid extraction, electrospray ionization mass spectrometry analysis of the active ethyl acetate fraction containing antifungal compounds revealed the production of 3-phenyllactic acid, cyclo-(L-Pro-L-Leu), cyclo-(L-Pro-L-Phe), and cyclo-(L-Phe-trans-4-OH-L-Pro). L. reuteri LR-92 has potential as a biocontrol agent for F. verticillioides and contributes to food safety.

Indexed as

FusariumLimosilactobacillus reuteriAntifungal AgentsBiological Control AgentsDiketopiperazinesAntifungal AgentsBiological Control Agentscyclo-(L-Pro-L-Phe)DiketopiperazinesAntifungal compoundsBiocontrolCyclic dipeptidesFungicidal and fungistatic activityMass spectrometry analysisPhenyllactic acid

Identifiers

PMID37531006
PMCPMC10484862
OpenAlexW4385477478

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.