ArticleeLife2023
scRNA-sequencing in chick suggests a probabilistic model for cell fate allocation at the neural plate border.
Article in eLife, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 28 citations in OpenAlex.
- Neural crest gene regulatory networks as drivers of development, diversification and disease.Nature reviews. Molecular cell biology · 2026Review
- Single-cell, clonal and spatial atlases of cranial placodes illuminate their specification and evolution.bioRxiv : the preprint server for biology · 2026Article
- A spatial and temporal atlas of tubulin isotype expression during neural crest EMT.bioRxiv : the preprint server for biology · 2026Article
- Dynamic and non-uniform expression of key transcription factors provides insights into the emergence of neural crest cells at the neural plate border.Development (Cambridge, England) · 2026Article
- Mechanical constraints on cell plasticity: insights from the olfactory epithelium.Frontiers in cell and developmental biology · 2026Review
- Integrated Single-cell Analysis Uncovers Regulatory Logic of Cranial Ectoderm Development.bioRxiv : the preprint server for biology · 2025Article
- Making sense of vertebrate senses from a neural crest and cranial placode evo-devo perspective.Trends in neurosciences · 2025Review
- Innovative Insights into Single-Cell Technologies and Multi-Omics Integration in Livestock and Poultry.International journal of molecular sciences · 2024Review
- Differentiating visceral sensory ganglion organoids from induced pluripotent stem cells.Nature methods · 2024Article
- A time-resolved single-cell roadmap of the logic driving anterior neural crest diversification from neural border to migration stages.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Adaptive introgression reveals the genetic basis of a sexually selected syndrome in wall lizards.Science advances · 2024Article
- In vitro induction of patterned branchial arch-like aggregate from human pluripotent stem cells.Nature communications · 2024Article
- In vitro modeling of cranial placode differentiation: Recent advances, challenges, and perspectives.Developmental biology · 2024Review
- The Foxi3 transcription factor is necessary for the fate restriction of placodal lineages at the neural plate border.Development (Cambridge, England) · 2023Article
- Maintenance of pluripotency-like signature in the entire ectoderm leads to neural crest stem cell potential.Nature communications · 2023Article
- Article
- Maintenance of pluripotency in the entire ectoderm enables neural crest formation.Research square · 2023Article
- Feedback Regulation of Signaling Pathways for Precise Pre-Placodal Ectoderm Formation in Vertebrate Embryos.Journal of developmental biology · 2022Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
The vertebrate 'neural plate border' is a transient territory located at the edge of the neural plate containing precursors for all ectodermal derivatives: the neural plate, neural crest, placodes and epidermis. Elegant functional experiments in a range of vertebrate models have provided an in-depth understanding of gene regulatory interactions within the ectoderm. However, these experiments conducted at tissue level raise seemingly contradictory models for fate allocation of individual cells. Here, we carry out single cell RNA sequencing of chick ectoderm from primitive streak to neurulation stage, to explore cell state diversity and heterogeneity. We characterise the dynamics of gene modules, allowing us to model the order of molecular events which take place as ectodermal fates segregate. Furthermore, we find that genes previously classified as neural plate border 'specifiers' typically exhibit dynamic expression patterns and are enriched in either neural, neural crest or placodal fates, revealing that the neural plate border should be seen as a heterogeneous ectodermal territory and not a discrete transitional transcriptional state. Analysis of neural, neural crest and placodal markers reveals that individual NPB cells co-express competing transcriptional programmes suggesting that their ultimate identify is not yet fixed. This population of 'border located undecided progenitors' (BLUPs) gradually diminishes as cell fate decisions take place. Considering our findings, we propose a probabilistic model for cell fate choice at the neural plate border. Our data suggest that the probability of a progenitor's daughters to contribute to a given ectodermal derivative is related to the balance of competing transcriptional programmes, which in turn are regulated by the spatiotemporal position of a progenitor.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.