ArticleiScience2023
Extensive intragenomic variation in the internal transcribed spacer region of fungi.
Article in iScience, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed.
- Challenges in ITS-based identification ofMicrobiology spectrum · 2026Article
- Taxonomic Identification of Two Novel Genera and Four Novel Species of Lipolytic Floral-Associated Yeasts.Journal of fungi (Basel, Switzerland) · 2026Article
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- A qPCR identification scheme to differentiate actinomycetoma from eumycetoma.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2026Article
- Genome-wide pervasiveness and localized variation of [Formula: see text]-mer-based genomic signatures in eukaryotes.Scientific reports · 2026Article
- Sampling Design and Sample Processing Affect Soil Biodiversity Assessments.Molecular ecology resources · 2026Article
- From soil to sequences: mechanisms and tools unravelling plant-rhizomicrobiome interactions.World journal of microbiology & biotechnology · 2026Review
- Remarkable recovery from aASM case reports · 2026Article
- Fungaemia Caused by the Rare Yeast Starmerella sorbosivorans: A Case Report with Identification via Molecular Phylogenetic Analysis and MALDI-TOF MS.Mycopathologia · 2026Article
- Population structure and genetic diversity of Phakopsora pachyrhizi in the Southeastern United States.G3 (Bethesda, Md.) · 2026Article
- Genetic-Geographic-Chemical Framework ofJournal of fungi (Basel, Switzerland) · 2026Article
- Another dark taxon comes to light:IMA fungus · 2026Article
- Basic assessment of microbial dynamics in large- and small-scale biofilters.Biotechnology reports (Amsterdam, Netherlands) · 2025Article
- High-quality assembly of the Chinese white truffle genome and recalibrated divergence time estimate provide insight into the evolutionary dynamics of Tuberaceae.Genome research · 2025Article
- Article
- The Khamkhains, Neurotrophic Drimane-Type Sesquiterpenoids Derived from a Polyporaceous Basidiomycete Originating from Thailand.Journal of natural products · 2025Article
- Powdery Mildew Caused by Leveillula taurica (Synonym: Phyllactinia taurica): A Global Challenge for Pepper Production.Molecular plant pathology · 2025Review
- A curated soil fungal dataset to advance fungal ecology and conservation research in Australia and Antarctica.Scientific data · 2025Article
- Deciphering the microbiota compositions of complex biofilms from hospital and domestic settings across Estonia, Germany, and the United Kingdom.FEMS microbiology letters · 2025Article
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Authors and funding
11 authors.
Funding
Abstract
Fungi are among the most biodiverse organisms in the world. Accurate species identification is imperative for studies on fungal ecology and evolution. The internal transcribed spacer (ITS) rDNA region has been widely accepted as the universal barcode for fungi. However, several recent studies have uncovered intragenomic sequence variation within the ITS in multiple fungal species. Here, we mined the genome of 2414 fungal species to determine the prevalence of intragenomic variation and found that the genomes of 641 species, about one-quarter of the 2414 species examined, contained multiple ITS copies. Of those 641 species, 419 (∼65%) contained variation among copies revealing that intragenomic variation is common in fungi. We proceeded to show how these copies could result in the erroneous description of hundreds of fungal species and skew studies evaluating environmental DNA (eDNA) especially when making diversity estimates. Additionally, many genomes were found to be contaminated, especially those of unculturable fungi.
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