Evidence map›Paper›PMID 37528254›Full record

ArticleStem cell reviews and reports2023

MSC-Derived Exosomes Ameliorate Intervertebral Disc Degeneration By Regulating the Keap1/Nrf2 Axis.

Guangyu Xu, Xiao Lu, Siyang Liu, Yuxuan Zhang, Shun Xu, Xiaosheng Ma, Xinlei Xia, Feizhou Lu, Fei Zou, Hongli Wang and 2 more

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In one paragraph

Article in Stem cell reviews and reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed, 1 pooled it
5.9field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 1 synthesis or guideline pooled it, 23 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 1 institution in 1 country.

Guangyu Xu *Department of Orthopedics, Huashan Hospital, Fudan University, Shanghai, 200040, China.
Xiao Lu *Department of Orthopedics, Huashan Hospital, Fudan University, Shanghai, 200040, China.
Siyang Liu *Department of Orthopedics, Huashan Hospital, Fudan University, Shanghai, 200040, China.
Yuxuan ZhangDepartment of Orthopedics, Huashan Hospital, Fudan University, Shanghai, 200040, China.
Shun XuDepartment of Orthopedics, Shanghai Fifth People's Hospital, Fudan University, Shanghai, 200240, China.
Xiaosheng MaDepartment of Orthopedics, Huashan Hospital, Fudan University, Shanghai, 200040, China.
Xinlei XiaDepartment of Orthopedics, Huashan Hospital, Fudan University, Shanghai, 200040, China.
Feizhou LuDepartment of Orthopedics, Huashan Hospital, Fudan University, Shanghai, 200040, China.
Fei ZouDepartment of Orthopedics, Huashan Hospital, Fudan University, Shanghai, 200040, China.
Hongli WangDepartment of Orthopedics, Huashan Hospital, Fudan University, Shanghai, 200040, China.
Jian SongDepartment of Orthopedics, Huashan Hospital, Fudan University, Shanghai, 200040, China. jsong16@fudan.edu.cn.ORCID 0000-0001-5850-025X
Jianyuan JiangDepartment of Orthopedics, Huashan Hospital, Fudan University, Shanghai, 200040, China. jianyuanjiang@sina.com.
Fudan University · CN

Funding

National Natural Science Foundation of China, China 81972093, 81972109, 82102620, 82172490 and 82272549Shanghai Sailing Program, Shanghai, Chin 20YF1429900
6 · The paper itself

Abstract

Bone marrow mesenchymal stem cell derived exosomes (BMSC-exos) are a crucial means of intercellular communication and can regulate a range of biological processes by reducing inflammation, decreasing apoptosis and promoting tissue repair. The process of intervertebral disc degeneration (IVDD) is accompanied by increased reactive oxygen species (ROS) because of a decrease in the expression of Nrf2, a critical transcription factor that resists excessive ROS. Our study demonstrated that BMSC-exos decreased ROS production by inhibiting Keap1 and promoting Nrf2 expression, attenuating the apoptosis, inflammation, and degeneration of nucelus pulposus (NP) cells. BMSC-exos promoted an increase in Nrf2 and nuclear translocation, while NF-κB expression was downregulated during this process. Additionally, the expression of antioxidative proteins was elevated after treatment with BMSC-exos. In vivo, we found more NP tissue retention in the BMSC-exos-treated group, along with more expression of Nrf2 and antioxidant-related proteins. Our findings demonstrated for the first time that BMSC-exos could restore the down-regulated antioxidant response system in degenerating NP cells by modulating the Keap1/Nrf2 axis. BMSC-exos could be used as an immediate ROS modulator in the treatment of intervertebral disc degeneration. When BMSC-exos were uptaken by NPCs, the expression of Keap1 decreased and this led to increased expression of Nrf2. Nuclear translocation of Nrf2 then promoted the synthesis of antioxidants against ROS and inhibited NF-kB signalling. Cellular inflammation, apoptosis, and ECM-related indicators were further reduced. Together, the process of IVDD was alleviated.

Indexed as

ExosomesIntervertebral Disc DegenerationKelch-Like ECH-Associated Protein 1Mesenchymal Stem CellsNF-E2-Related Factor 2AnimalsApoptosisHumansMaleNF-kappa BNucleus PulposusRatsRats, Sprague-DawleyReactive Oxygen SpeciesSignal TransductionKelch-Like ECH-Associated Protein 1NF-E2-Related Factor 2NF-kappa BReactive Oxygen SpeciesExosomesInflammationLow back painNrf2Oxidative stress

Identifiers

PMID37528254
OpenAlexW4385446250

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.