ArticleLight, science & applications2023
Unveiling the lamellar structure of the human cornea over its full thickness using polarization-resolved SHG microscopy.
Article in Light, science & applications, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 32 citations in OpenAlex.
- Role of Neural Crest Cells in Establishing Corneal Transparency During Embryonic Development in Mice.Journal of cellular physiology · 2026Article
- Refractive Index Engineering for Human Corneal Tissue by Femtosecond Laser Two-Photon Cross-Linking.Journal of biophotonics · 2026Article
- Evaluating the applicability of computational super-resolution techniques for harmonic generation microscopy.Scientific reports · 2026Article
- Global spatiotemporal biomechanics using video swin transformer: multiscale validation and clinical impact for keratoconus suspects.NPJ digital medicine · 2026Article
- Advanced Corneal Hydrogels: From Passive Replacement to Active Regeneration and Intelligent Interaction.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Label-free mid-infrared dichroism-sensitive photoacoustic microscopy for histostructural analysis of engineered heart tissues.Light, science & applications · 2026Article
- Collagen Mechanics.Sub-cellular biochemistry · 2026Review
- Optical characterization of ocular collagen microstructure and anisotropy in animal and human eyes.Biomedical optics express · 2026Article
- Collagen microarchitecture from polarized light imaging: a biomechanics perspective.Journal of biomedical optics · 2026Review
- Enhanced magnetic second-harmonic generation in an ultra-compact plasmonic nanocavity.Light, science & applications · 2025Article
- Article
- Characterising corneal changes in aniridia-related keratopathy using in vivo confocal microscopy and a self-supervised AI model.BMJ open ophthalmology · 2025Observational
- 4D-Spatiotemporal SHG Imaging for the Analysis of Drug-Induced Changes in the Dura Mater.Analytical chemistry · 2025Article
- Quantitative Assessment of Collagen Remodeling during a Murine Pregnancy.ACS photonics · 2024Article
- Widefield polarization-resolved second harmonic generation imaging of entire thyroid nodule sections for the detection of capsular invasion.Biomedical optics express · 2024Article
- Lamina Cribrosa Insertions Into the Sclera Are Sparser, Narrower, and More Slanted in the Anterior Lamina.Investigative ophthalmology & visual science · 2024Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
A key property of the human cornea is to maintain its curvature and consequently its refraction capability despite daily changes in intraocular pressure. This is closely related to the multiscale structure of the corneal stroma, which consists of 1-3 µm-thick stacked lamellae made of thin collagen fibrils. Nevertheless, the distribution, size, and orientation of these lamellae along the depth of the cornea are poorly characterized up to now. In this study, we use second harmonic generation (SHG) microscopy to visualize the collagen distribution over the full depth of 10 intact and unstained human corneas (500-600 µm thick). We take advantage of the small coherence length in epi-detection to axially resolve the lamellae while maintaining the corneal physiological curvature. Moreover, as raw epi-detected SHG images are spatially homogenous because of the sub-wavelength size of stromal collagen fibrils, we use a polarimetric approach to measure the collagen orientation in every voxel. After a careful validation of this approach, we show that the collagen lamellae (i) are mostly oriented along the inferior-superior axis in the anterior stroma and along the nasal-temporal axis in the posterior stroma, with a gradual shift in between and (ii) exhibit more disorder in the anterior stroma. These results represent the first quantitative characterization of the lamellar structure of the human cornea continuously along its entire thickness with micrometric resolution. It also shows the unique potential of P-SHG microscopy for imaging of collagen distribution in thick dense tissues.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.