Evidence map›Paper›PMID 37526868›Full record

ArticleBiotechnology letters2023

High-efficiency genetic engineering toolkit for virus based on lambda red-mediated recombination.

Jing Yi, Maifei Zhang, Lin Zhu, Changzhi Xu, Binglin Li, Panpan Wu, Hang Wu, Buchang Zhang

Abstract read
PubMed Publisher
In one paragraph

Article in Biotechnology letters, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Jing Yi *School of Life Sciences, Institute of Physical Science and Information Technology, Anhui University, 111 Jiulong Road, Hefei, 230601, Anhui, People's Republic of China.
Maifei Zhang *School of Life Sciences, Institute of Physical Science and Information Technology, Anhui University, 111 Jiulong Road, Hefei, 230601, Anhui, People's Republic of China.
Lin Zhu *School of Life Sciences, Institute of Physical Science and Information Technology, Anhui University, 111 Jiulong Road, Hefei, 230601, Anhui, People's Republic of China.
Changzhi XuSchool of Life Sciences, Institute of Physical Science and Information Technology, Anhui University, 111 Jiulong Road, Hefei, 230601, Anhui, People's Republic of China.
Binglin LiSchool of Life Sciences, Institute of Physical Science and Information Technology, Anhui University, 111 Jiulong Road, Hefei, 230601, Anhui, People's Republic of China.
Panpan WuSchool of Life Sciences, Institute of Physical Science and Information Technology, Anhui University, 111 Jiulong Road, Hefei, 230601, Anhui, People's Republic of China.
Hang WuSchool of Life Sciences, Institute of Physical Science and Information Technology, Anhui University, 111 Jiulong Road, Hefei, 230601, Anhui, People's Republic of China. wuhang@ahu.edu.cn.ORCID http://orcid.org/0000-0003-2724-7065
Buchang ZhangSchool of Life Sciences, Institute of Physical Science and Information Technology, Anhui University, 111 Jiulong Road, Hefei, 230601, Anhui, People's Republic of China.
Anhui University · CN

Funding

Anhui Provincial Natural Science Foundation 2208085Y09National Natural Science Foundation of China 31972930National Natural Science Foundation of China 32170073Natural Science Research Project of Colleges and Universities in Anhui Province 2022AH050063Natural Science Research Project of Colleges and Universities in Anhui Province KJ2021A0077
6 · The paper itself

Abstract

purposeViruses, such as Ebola virus (EBOV), evolve rapidly and threaten the human health. There is a great demand to exploit efficient gene-editing techniques for the identification of virus to probe virulence mechanism for drug development.

methodsBased on lambda Red recombination in Escherichia coli (E. coli), counter-selection, and in vitro annealing, a high-efficiency genetic method was utilized here for precisely engineering viruses. EBOV trVLPs assay and dual luciferase reporter assay were used to further test the effect of mutations on virus replication.

resultsConsidering the significance of matrix protein VP24 in EBOV replication, the types of mutations within vp24, including several single-base substitutions, one double-base substitution, two seamless deletions, and one targeted insertion, were generated on the multi-copy plasmid of E. coli. Further, the length of the homology arms for recombination and in vitro annealing, and the amount of DNA cassettes and linear plasmids were optimized to create a more elaborate and cost-efficient protocol than original approach. The effects of VP24 mutations on the expression of a reporter gene (luciferase) from the EBOV minigenome were determined, and results indicated that mutations of key sites within VP24 have significant impacts on EBOV replication.

conclusionThis precise mutagenesis method will facilitate effective and simple editing of viral genes in E. coli.

Indexed as

EbolavirusHemorrhagic Fever, EbolaEscherichia coliGenetic EngineeringHumansRecombination, GeneticViral ProteinsViral ProteinsEbola virusEscherichia coliRedαβVirus editingVP24

Identifiers

PMID37526868
OpenAlexW4385442867

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.