ArticleJournal of the American Society for Mass Spectrometry2023
Aziridination-Assisted Mass Spectrometry of Nonpolar Sterol Lipids with Isomeric Resolution.
Article in Journal of the American Society for Mass Spectrometry, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 15 citations in OpenAlex.
- Mapping Double-Bond Positional Isomers of Phosphatidylcholines in Zebrafish Using OzMALDI Mass Spectrometry Imaging.Journal of the American Society for Mass Spectrometry · 2026Article
- Aziridination-Enabled Structural and Quantitative Lipidomics.Journal of the American Society for Mass Spectrometry · 2026Review
- Making Ozonolysis Mass Spectrometry More Accessible: A Simple LC-OzESI-MS Workflow for Carbon-Carbon Double Bond Position Elucidation in Lipids and Natural Products.Journal of the American Society for Mass Spectrometry · 2026Article
- Phospholipid Profiling Established by Structure-Rich Fragments for Molecular Species Level Shotgun Analysis.Rapid communications in mass spectrometry : RCM · 2026Article
- Oxygen attachment dissociation (OAD) MS/MS in the identification of positional isomers of dysregulated lipids detected in an ethanol exposure metabolomics study in mice.Metabolomics : Official journal of the Metabolomic Society · 2025Article
- Interfacial electromigration for accelerated reactions.Analytica chimica acta · 2025Article
- Bifunctional Tagging through N-Doped Ozonide for Charge Switching and Isomeric Characterization of Glycerophospholipids Using Tandem Mass Spectrometry.Analytical chemistry · 2025Article
- OzMALDI: A Gas-Phase, In-Source Ozonolysis Reaction for Efficient Double-Bond Assignment in Mass Spectrometry Imaging with Matrix-Assisted Laser Desorption/Ionization.Analytical chemistry · 2025Article
- OzNOxESI: A Novel Mass Spectrometry Ion Chemistry for Elucidating Lipid Double-Bond Regioisomerism in Complex Mixtures.Analytical chemistry · 2025Article
- [Progress in applications of ambient ionization mass spectrometry for lipids identification].Se pu = Chinese journal of chromatography · 2025Review
- Mapping Lipid C═C Isomer Profiles of Human Gut Bacteria through a Novel Structural Lipidomics Workflow Assisted by Chemical Epoxidation.Analytical chemistry · 2024Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Characterization of nonpolar lipids is crucial due to their essential biological functions and ability to exist in various isomeric forms. In this study, we introduce the N-H aziridination method to target carbon-carbon double bonds (C═C bonds) in nonpolar sterol lipids. The resulting fragments are readily dissociated upon collision-induced dissociation, generating specific fragment ions for C═C bond position determination and fingerprint fragments for backbone characterization. This method significantly enhances lipid ionization efficiency, thereby improving the sensitivity and accuracy of nonpolar lipid analysis. We demonstrated that aziridination of sterols leads to distinctive fragmentation pathways for chain and ring C═C bonds, enabling the identification of sterol isomers such as desmosterol and 7-dehydrocholesterol. Furthermore, aziridination can assist in identifying the sterol backbone by providing fingerprint tandem mass spectra. We also demonstrated the quantitative capacity of this approach with a limit of detection of 10 nM in the solvent mixture of methanol and water. To test the feasibility of this method in complex biological samples, we used mouse prostate cancerous tissues and found significant differences in nonpolar lipid profiles between healthy and cancerous samples. The high efficiency and specificity of aziridination-assisted mass spectrometric analysis, as well as its quantitative analysis ability, make it highly suitable for broad applications in nonpolar lipid research.
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