Evidence map›Paper›PMID 37522925›Full record

ArticleJournal of cancer research and clinical oncology2023

Liquid nitrogen frozen cells for chemotherapy drug delivery and vaccination of melanoma.

Minliang Wu, Yuai Xiao, Jianguo Huang, Yuchong Wang, Yifan Zhang, Jianguo Xu, Haiying Dai, Chuan Lv, Yihui Hu, Bingdi Chen and 3 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of cancer research and clinical oncology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 3 institutions in 1 country.

Minliang Wu *Department of Plastic Surgery, Changhai Hospital, Naval Medical University, Shanghai, 200433, China.
Yuai Xiao *Department of Plastic Surgery, Changhai Hospital, Naval Medical University, Shanghai, 200433, China.
Jianguo Huang *Institute for Regenerative Medicine, Shanghai East Hospital, The Institute for Biomedical Engineering & Nano Science, School of Medicine, Tongji University, Shanghai, 200092, China.
Yuchong WangDepartment of Plastic Surgery, Changhai Hospital, Naval Medical University, Shanghai, 200433, China.
Yifan ZhangInstitute for Regenerative Medicine, Shanghai East Hospital, The Institute for Biomedical Engineering & Nano Science, School of Medicine, Tongji University, Shanghai, 200092, China.
Jianguo XuDepartment of Plastic Surgery, Changhai Hospital, Naval Medical University, Shanghai, 200433, China.
Haiying DaiDepartment of Plastic Surgery, Changhai Hospital, Naval Medical University, Shanghai, 200433, China.
Chuan LvDepartment of Plastic Surgery, Changhai Hospital, Naval Medical University, Shanghai, 200433, China.
Yihui HuInstitute for Regenerative Medicine, Shanghai East Hospital, The Institute for Biomedical Engineering & Nano Science, School of Medicine, Tongji University, Shanghai, 200092, China.
Bingdi ChenInstitute for Regenerative Medicine, Shanghai East Hospital, The Institute for Biomedical Engineering & Nano Science, School of Medicine, Tongji University, Shanghai, 200092, China.
Qingge FuDepartment of Orthopedic Trauma, Changhai Hospital, Naval Medical University, Shanghai, 200433, China. fqg7821@163.com.
Wenjun LeInstitute for Regenerative Medicine, Shanghai East Hospital, The Institute for Biomedical Engineering & Nano Science, School of Medicine, Tongji University, Shanghai, 200092, China. wenjunle@tongji.edu.cn.
Chunyu XueDepartment of Plastic Surgery, Changhai Hospital, Naval Medical University, Shanghai, 200433, China. xcyfun@sina.com.
Second Military Medical University · CNShanghai East Hospital · CNChanghai Hospital · CN

Funding

Fundamental Research Funds for the Central Universities 22120210583National Natural Science Foundation of China 82002843Natural Science Foundation of Shanghai 21ZR1479100Sichuan Science and Technology Program 2020YFSY0030
6 · The paper itself

Abstract

purposeCancer vaccine (CV) has thrived as a promising tool for cancer prevention and treatment. However, how to maintain the integrity and diversity of individualized vaccine antigens and activate the adaptive immune system is still challenging.

methodsHerein, a preventive and therapeutic vaccine platform for in situ effective multi-model synergistic therapy is developed. In our study, we process B16F10 cells by liquid nitrogen frozen (LNF) to obtain LNF cells, the characterization of LNF cells were conducted. Moreover,  the anti-tumor effect and immune activation ability were studied, and the role as a CV were investigated.

resultsThe LNF cells preserve intact cellular structure and tumor-associated self-antigen gp100. Moreover, LNF cells have the ability of loading and releasing doxorubicin (DOX). Except for the anti-tumor effect of chemotherapy brought by DOX, the LNF cells can promote the maturation of dendritic cells (DCs) and induce immune response by activating CD4

conclusionIn this work, we obtain the LNF cells with a simple procedure. The LNF cells not only provides a tumor cells-based multi-modal system for cancer therapy but inspires new insights into future development of individualized CVs strategies. This study processes live B16F10 cells by liquid nitrogen frozen to obtain LNF cells, which preserve cell integrity and homologous targeting ability. The LNF cells can load and deliver drug and can serve as tumor vaccine. Results demonstrated the LNF cells have effective prophylactic ability, and ideal anti-tumor ability with the loaded drug and adjuvant.

Indexed as

ChemotherapyDrug deliveryLiquid nitrogen frozenMelanomaVaccine

Identifiers

PMID37522925
PMCPMC11798233
OpenAlexW4385406789

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.