Evidence map›Paper›PMID 37521362›Full record

ArticleBrazilian journal of veterinary medicine2023

Molecular detection of porcine circovirus (PCV2 and PCV3), torque teno swine virus 1 and 2 (TTSuV1 and TTSuVk2), and histopathological findings in swine organs submitted to regular slaughter in Southeast, Brazil.

Amanda Eduarda de Souza, Ana Claudia de Menezes Cruz, Ingrid Lyrio Rodrigues, Eulógio Carlos Queiroz de Carvalho, Rafael Brandão Varella, Raphael Mansur Medina, Rachel Bittencourt Ribeiro Rodrigues, Renato Luiz Silveira, Tatiana Xavier de Castro

Open access · goldAbstract read
In one paragraph

Article in Brazilian journal of veterinary medicine, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.4field-weighted citation impact, top 44% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 2 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 5 institutions in 1 country.

Amanda Eduarda de SouzaVeterinarian, Programa de Pós-Graduação em Microbiologia e Parasitologia Aplicadas (PPGMPA), Departamento de Microbiologia e Parasitologia (MIP), Universidade Federal Fluminense (UFF). Niterói, RJ. Brazil.ORCID 0000-0003-2327-3864
Ana Claudia de Menezes CruzVeterinarian, MSc. PPGMPA, MIP, UFF. Niterói, RJ. Brazil.ORCID 0000-0002-4520-6884
Ingrid Lyrio RodriguesVeterinarian, MSc. PPGMPA, MIP, UFF. Niterói, RJ. Brazil.ORCID 0000-0002-3250-2385
Eulógio Carlos Queiroz de CarvalhoVeterinarian, DSc. Universidade Estadual Norte Fluminense (UENF), Campos dos Goytacazes, RJ, Brazil.ORCID 0000-0002-1785-8578
Rafael Brandão VarellaVeterinarian, DSc. MIP, UFF. Niterói, RJ. Brazil.ORCID 0000-0002-1698-5989
Raphael Mansur MedinaVeterinarian, MSc. Autonomus, Campos dos Goytacazes, RJ, Brazil.ORCID 0000-0003-4514-2160
Rachel Bittencourt Ribeiro RodriguesVeterinarian, MSc. Autonomus, Campos dos Goytacazes, RJ, Brazil.ORCID 0000-0002-4493-5372
Renato Luiz SilveiraVeterinarian, DSc. Departamento de Morfologia (MMO), UFF. Niterói, RJ. Brazil.ORCID 0000-0001-7876-0761
Tatiana Xavier de CastroVeterinarian, DSc. MIP, UFF. Niterói, RJ. Brazil.ORCID 0000-0002-2772-6405
Universidade Federal Fluminense · BRPrefeitura Municipal de Campos dos Goytacazes · BRAssociação Brasileira de Oncologia Veterinária · BRFundação de Estudo e Pesquisa em Medicina Veterinária e Zootecnia · BRState University of Norte Fluminense · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Porcine circovirus 2 and 3 (PCV2 and PCV3) and torque teno sus virus 1 and 2 (TTSuV1 and TTSuVk2) are important pathogens in pig associated with post-weaning mortality, different clinical syndromes in adults (PCVAD), and a decrease of average daily weight gain (PCV2-SI) but little is known about the infection on asymptomatic pigs. The aim of this study was to evaluate the presence of PCV2, PCV3, TTSuV1, and TTSuVk2 in swine organ samples from asymptomatic pigs slaughtered in Espírito Santo State, South-eastern Brazil, through molecular detection and histopathological analysis. Nested PCR showed the presence of PCV2 DNA in 10% (14/140), PCV3 in 13.6% (19/140), TTSuV1 in 12.9% (18/140), and TTSuVk2 in 30% (42/140) of the tissue samples. All four viruses were detected in the lung, kidney, lymph node, and liver. TTSuVk2 was detecded in 30% (42/140), PCV3 in 13.6% (19/140), TTSuV1 in 12.9% (18/140), and PCV2 in 10% (14/140) of the samples. Single infections were observed in 30.7% (43/140), while co-detections in the same tissue occurred in 15.7% (22/140). The most frequent combinations were TTSuV1/TTSuVk2 in 31.8% (7/22), PCV2/TTSuVk2 in 18.1% (4/22), and PCV2/PCV3/TTSuVk2 in 13.6% (3/22). Lymphocyte depletion was associated with TTSuVk2 infection (p = 0.0041) suggesting that TTSuVK2 plays an induction of PMWS-like lymphoid lesions in pigs. The data obtained in this study show that PCV2, PCV3, TTSuV1, and TTSuVk2 are related to infection in asymptomatic animals with different tissue lesions, and the molecular diagnosis for these pathogens should be considered in the sanitary monitoring of herds.

Indexed as

asymptomatic infectioncoinfectionHistopathologynested PCRpig

Identifiers

PMID37521362
PMCPMC10374291
OpenAlexW4385270815

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.