ArticleMetabolites2023
Real-Time Quantitative PCR: Primer Design, Reference Gene Selection, Calculations and Statistics.
Article in Metabolites, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed.
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- Local Zoledronate Administration Modulates Periapical Lesion Development in Immunologically Distinct Rat Strains.Dentistry journal · 2026Article
- Staphylococcal Cassette ChromosomeAntibiotics (Basel, Switzerland) · 2026Article
- Functional Exploration of African Colorectal Cancer Patients Using PersonalisedbioRxiv : the preprint server for biology · 2026Article
- Investigating the Effects of Sea Anemone (Stichodactyla haddoni) Toxin on Potassium and Sodium Channel Gene Expression and Cell Death Mechanisms in A549 Cells.Marine biotechnology (New York, N.Y.) · 2026Article
- Application and prospects of veterinary diagnostic techniques in laboratory monkeys.Frontiers in veterinary science · 2026Review
- Comparative analysis of skin transcriptome reveals differences of cashmere fineness in different body parts of Inner Mongolia cashmere goats.Animal bioscience · 2025Article
- Development of a TaqMan based real-time PCR assay for the detection of bovine ephemeral fever virus.Molecular biology reports · 2025Article
- Selection and validation of RT-qPCR reference genes for multi-tissue gene expression normalization in two honeybee subspecies across post-emergence developmental stages.BMC genomics · 2025Article
- MTA-TST Axis-Mediated Apoptosis Activation: A Multi-Omics Insight Into High-Protein Diet's Anti-Adiposity Effect.Food science & nutrition · 2025Article
- Selection of reference genes in Thermothelomyces fergusii: insights from genomic and expression analysis in response to different carbon sources.BMC genomics · 2025Article
- Cattle Zoonotic and Non-Zoonotic Tick-Borne Pathogens in Europe-A Retrospective Analysis of the Past 15 Years.Animals : an open access journal from MDPI · 2025Review
- Article
- The coadministration of Lactobacillus probiotic augments the antitumor effect of telmisartan in rats.AMB Express · 2025Article
- Selection and validation of reference genes for quantitative real-time PCR during the developmental stages of seeds inFrontiers in plant science · 2025Article
- Genomic survey and evolution analysis of calcium-dependent protein kinases in plants and their stress-responsive patterns in populus.BMC genomics · 2024Article
- Characterization, evolution, and abiotic stress responses of leucine-rich repeat receptor-like protein kinases (LRR-RLK) in Liriodendron chinense.BMC genomics · 2024Article
- The Impact of Next-Generation Sequencing Added to Multiplex PCR on Antibiotic Stewardship in Critically Ill Patients with Suspected Pneumonia.Diagnostics (Basel, Switzerland) · 2024Article
- Advances in the detection technology of vegetable soil borne fungi and bacteria.Frontiers in microbiology · 2024Review
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Real-time quantitative PCR is a technique that can measure the content of the target nucleic acid sequence of interest in a given sample. It is mainly divided into absolute and relative quantitative methods. The relative quantification is mainly used in gene expressions for functional genomic and transcriptome studies. However, to use this technology accurately, there are some key points to master. First, specific primers need to be designed to ensure amplification of the gene of interest (GOI). Second, the appropriate reference gene or reference gene combination has to be selected. Finally, scientific gene expression level calculations and statistics are required to obtain accurate results. Therefore, this work proposes a workflow for relative quantitative PCR and introduces the relevant points so that beginners can better understand and use this technology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.