Evidence map›Paper›PMID 37511146›Full record

ArticleInternational journal of molecular sciences2023

Effects of Serotonin on Cell Viability, Permeability of Bovine Mammary Gland Epithelial Cells and Their Transcriptome Analysis.

Jie Lu, Guohao Huang, Xuan Chang, Bingni Wei, Yawang Sun, Zhengguo Yang, Yongju Zhao, Zhongquan Zhao, Guozhong Dong, Juncai Chen

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.1field-weighted citation impact, top 53% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 1 citations in OpenAlex.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Jie LuCollege of Animal Science and Technology, Southwest University, Chongqing 400715, China.
Guohao HuangCollege of Animal Science and Technology, Southwest University, Chongqing 400715, China.
Xuan ChangCollege of Animal Science and Technology, Southwest University, Chongqing 400715, China.
Bingni WeiCollege of Animal Science and Technology, Southwest University, Chongqing 400715, China.
Yawang SunCollege of Animal Science and Technology, Southwest University, Chongqing 400715, China.
Zhengguo YangCollege of Animal Science and Technology, Southwest University, Chongqing 400715, China.
Yongju ZhaoCollege of Animal Science and Technology, Southwest University, Chongqing 400715, China.ORCID 0000-0001-9256-8856
Zhongquan ZhaoCollege of Animal Science and Technology, Southwest University, Chongqing 400715, China.
Guozhong DongCollege of Animal Science and Technology, Southwest University, Chongqing 400715, China.ORCID 0000-0001-7355-3050
Juncai ChenCollege of Animal Science and Technology, Southwest University, Chongqing 400715, China.ORCID 0000-0003-3942-3759
Southwest University · CN

Funding

Chongqing Municipal Training Program of Innovation and Entrepreneurship for Undergraduates S202210635056Fundamental Research Funds for the Central Universities SWU-KW22001Natural Science Foundation of Chongqing CSTB2022NSCQ-MSX1319Science and Technology Research Program of Chongqing Municipal Education Commission KJQN202200203
6 · The paper itself

Abstract

Serotonin (5-HT) has been reported to play an important role in mammary gland involution that is defined as the process through which the gland returns to a nonlactating state. However, the overall picture of the regulatory mechanisms of 5-HT and the effects of serotonylation on mammary gland involution still need to be further investigated. The current study aimed to investigate the effects of 5-HT on global gene expression profiles of bovine mammary epithelial cells (MAC-T) and to preliminarily examine whether the serotonylation involved in the mammary gland involution by using Monodansylcadaverine (MDC), a competitive inhibitor of transglutaminase 2. Results showed that a high concentration of 5-HT decreased viability and transepithelial electrical resistance (TEER) in MAC-T cells. Transcriptome analysis indicated that 2477 genes were differentially expressed in MAC-T cells treated with 200 μg/mL of 5-HT compared with the control group, and the Notch, p53, and PI3K-Akt signaling pathways were enriched. MDC influenced 5-HT-induced MAC-T cell death, fatty acid synthesis, and the formation and disruption of tight junctions. Overall, a high concentration of 5-HT is able to accelerate mammary gland involution, which may be regulated through the Notch, p53, and PI3K-Akt signaling pathways. Serotonylation is involved in bovine mammary gland involution.

Indexed as

LactationSerotoninAnimalsCattleCell SurvivalEpithelial CellsFemaleGene Expression ProfilingMammary Glands, AnimalPermeabilityPhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktTumor Suppressor Protein p53Phosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktSerotoninTumor Suppressor Protein p53cell viabilityMAC-T cellsmammary gland involutionserotonylationtight junctiontranscriptome profiles

Identifiers

PMID37511146
PMCPMC10379418
OpenAlexW4384202440

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.