Evidence map›Paper›PMID 37508528›Full record

ArticleCells2023

Perfusion and Ultrasonication Produce a Decellularized Porcine Whole-Ovary Scaffold with a Preserved Microarchitecture.

Gustavo Henrique Doná Rodrigues Almeida, Leandro Norberto da Silva-Júnior, Mariana Sversut Gibin, Henrique Dos Santos, Bianca de Oliveira Horvath-Pereira, Leticia Beatriz Mazo Pinho, Mauro Luciano Baesso, Francielle Sato, Luzmarina Hernandes, Charles R Long and 3 more

Open access · goldAbstract read
In one paragraph

Article in Cells, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
7.6field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 24 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 3 institutions in 2 countries.

Gustavo Henrique Doná Rodrigues AlmeidaDepartment of Surgery, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo 05508-270, Brazil.ORCID 0000-0003-2640-8250
Leandro Norberto da Silva-JúniorDepartment of Surgery, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo 05508-270, Brazil.ORCID 0000-0003-2628-8746
Mariana Sversut GibinDepartment of Physics, State University of Maringá, Maringá 87020-900, Brazil.ORCID 0000-0002-4575-2703
Henrique Dos SantosDepartment of Physics, State University of Maringá, Maringá 87020-900, Brazil.ORCID 0000-0002-6527-5844
Bianca de Oliveira Horvath-PereiraDepartment of Surgery, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo 05508-270, Brazil.
Leticia Beatriz Mazo PinhoDepartment of Surgery, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo 05508-270, Brazil.
Mauro Luciano BaessoDepartment of Physics, State University of Maringá, Maringá 87020-900, Brazil.ORCID 0000-0001-6017-2582
Francielle SatoDepartment of Physics, State University of Maringá, Maringá 87020-900, Brazil.ORCID 0000-0002-8273-2891
Luzmarina HernandesDepartment of Morphological Sciences, State University of Maringa, Maringá 87020-900, Brazil.ORCID 0000-0003-4284-4394
Charles R LongDepartment of Veterinary Physiology and Pharmacology, School of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, TX 77843, USA.
Luciana RellyDepartment of Veterinary Physiology and Pharmacology, School of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, TX 77843, USA.
Maria Angelica MiglinoDepartment of Surgery, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo 05508-270, Brazil.
Ana Claudia Oliveira CarreiraDepartment of Surgery, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo 05508-270, Brazil.
Universidade de São Paulo · BRUniversidade Estadual de Maringá · BRTexas A&M University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The application of decellularized scaffolds for artificial tissue reconstruction has been an approach with great therapeutic potential in regenerative medicine. Recently, biomimetic ovarian tissue reconstruction was proposed to reestablish ovarian endocrine functions. Despite many decellularization methods proposed, there is no established protocol for whole ovaries by detergent perfusion that is able to preserve tissue macro and microstructure with higher efficiency. This generated biomaterial may have the potential to be applied for other purposes beyond reproduction and be translated to other areas in the tissue engineering field. Therefore, this study aimed to establish and standardize a protocol for porcine ovaries' decellularization based on detergent perfusion and ultrasonication to obtain functional whole-ovary scaffolds. For that, porcine ovaries (

Indexed as

OvaryTissue ScaffoldsAnimalsDetergentsExtracellular MatrixFemaleMicePerfusionSwineDetergentsdecellularizationovariesperfusionreproductionscaffoldtissue engineering

Identifiers

PMID37508528
PMCPMC10378497
OpenAlexW4384557714

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.