ArticleNPJ biofilms and microbiomes2023
Distinct types of multicellular aggregates in Pseudomonas aeruginosa liquid cultures.
Article in NPJ biofilms and microbiomes, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 24 citations in OpenAlex.
- Modeling Multiscale Architecture of Biofilm Extracellular Matrix and Its Role in Oxygen Transport.Biotechnology and bioengineering · 2026Article
- The impact of inoculation methods on bacterial aggregation and antimicrobial susceptibility testing.Microbiology (Reading, England) · 2026Article
- Raman spectroscopy reveals growth phase-dependent molecular differences in bacterial membrane vesicles.Journal of bacteriology · 2026Article
- Spatial structure: shaping the ecology and evolution of microbial communities.FEMS microbiology reviews · 2026Review
- AFM characterization of earlymSystems · 2025Article
- Interaction of Bacteria and Fleas, Focusing on the Plague Bacterium-A Review.Microorganisms · 2025Review
- Complementary killing activities ofAntimicrobial agents and chemotherapy · 2025Article
- Article
- Bacterial aggregation triggered by low-level antibiotic-mediated lysis.NPJ biofilms and microbiomes · 2024Article
- Physiology, fast and slow: bacterial response to variable resource stoichiometry and dilution rate.mSystems · 2024Article
- Marine bacteriamBio · 2024Article
- Collective peroxide detoxification determines microbial mutation rate plasticity in E. coli.PLoS biology · 2024Article
- Multitasking functions of bacterial extracellular DNA in biofilms.Journal of bacteriology · 2024Review
- Divergent molecular strategies drive evolutionary adaptation to competitive fitness in biofilm formation.The ISME journal · 2024Article
- Engineered plastic-associated bacteria for biodegradation and bioremediation.Biotechnology for the environment · 2024Review
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Authors and funding
7 authors at 6 institutions in 4 countries.
Funding
Abstract
Pseudomonas aeruginosa forms suspended multicellular aggregates when cultured in liquid media. These aggregates may be important in disease, and/or as a pathway to biofilm formation. The polysaccharide Psl and extracellular DNA (eDNA) have both been implicated in aggregation, but previous results depend strongly on the experimental conditions. Here we develop a quantitative microscopy-based method for assessing changes in the size distribution of suspended aggregates over time in growing cultures. For exponentially growing cultures of P. aeruginosa PAO1, we find that aggregation is mediated by cell-associated Psl, rather than by either eDNA or secreted Psl. These aggregates arise de novo within the culture via a growth process that involves both collisions and clonal growth, and Psl non-producing cells do not aggregate with producers. In contrast, we find that stationary phase (overnight) cultures contain a different type of multicellular aggregate, in which both eDNA and Psl mediate cohesion. Our findings suggest that the physical and biological properties of multicellular aggregates may be very different in early-stage vs late-stage bacterial cultures.
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Registered trials
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