Evidence map›Paper›PMID 37506208›Full record

ArticleScience advances2023

Lectin-Seq: A method to profile lectin-microbe interactions in native communities.

Robert L McPherson, Christine R Isabella, Rebecca L Walker, Dallis Sergio, Sunhee Bae, Tony Gaca, Smrithi Raman, Le Thanh Tu Nguyen, Darryl A Wesener, Melanie Halim and 12 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Science advances, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed, 1 pooled it
7.5field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 1 synthesis or guideline pooled it, 25 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Article
  4. Review
  5. Article
  6. Review
  7. Review
  8. Review
  9. Article
  10. Article
  11. Defined Glycan Ligands for Detecting Rare l-Sugar-Binding Proteins.Journal of the American Chemical Society · 2025
    Article
  12. Review
  13. Article
  14. Article
  15. Soluble Human Lectins at the Host-Microbe Interface.Annual review of biochemistry · 2024
    Review
  16. Chemical biology tools to probe bacterial glycans.Current opinion in chemical biology · 2024
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

22 authors at 4 institutions in 1 country.

Robert L McPhersonDepartment of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.ORCID 0000-0002-5922-889X
Christine R IsabellaDepartment of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.ORCID 0000-0003-0786-7240
Rebecca L WalkerThe Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.ORCID 0000-0003-1977-9547
Dallis SergioThe Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.ORCID 0000-0001-9290-7088
Sunhee BaeDepartment of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.ORCID 0000-0002-8913-9476
Tony GacaThe Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Smrithi RamanDepartment of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Le Thanh Tu NguyenCenter for Microbiome Informatics and Therapeutics, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.ORCID 0000-0001-7012-1121
Darryl A WesenerEdison Family Center for Genome Sciences & Systems Biology, Washington University School of Medicine, Saint Louis, MO 63110, USA.ORCID 0000-0002-8132-7018
Melanie HalimDepartment of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Michael G WuoDepartment of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.ORCID 0000-0001-5001-7538
Amanda DuganDepartment of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Robert KerbyDepartment of Bacteriology, University of Wisconsin-Madison, Madison, WI 53706, USA.
Soumi GhoshDepartment of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.ORCID 0000-0001-6101-0147
Federico E ReyDepartment of Bacteriology, University of Wisconsin-Madison, Madison, WI 53706, USA.ORCID 0000-0002-0239-7780
Catherine DhennezelThe Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.ORCID 0000-0003-3273-7165
Gleb PishchanyThe Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.ORCID 0000-0001-9303-010X
Valerie LenschDepartment of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.ORCID 0000-0003-0371-2866
Hera VlamakisCenter for Microbiome Informatics and Therapeutics, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.ORCID 0000-0003-1086-9191
Eric J AlmCenter for Microbiome Informatics and Therapeutics, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.ORCID 0000-0001-8294-9364
Ramnik J XavierCenter for Microbiome Informatics and Therapeutics, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.ORCID 0000-0002-5630-5167
Laura L KiesslingDepartment of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.ORCID 0000-0001-6829-1500
Massachusetts Institute of Technology · USBroad Institute · USUniversity of Wisconsin–Madison · USWashington University in St. Louis · US

Funding

Pilot & Feasibility ProgramP30DK043351 · NIDDK · MASSACHUSETTS GENERAL HOSPITAL · PI RAYMOND T CHUNG · 1991 to 2026
$35.3M
Synthetic Ligands for Modulating Immune Cell ResponsesR01AI055258 · NIAID · UNIVERSITY OF WISCONSIN-MADISON · PI KIESSLING, LAURA L · 2003 to 2024
$7.8M
Establishing mechanistic links between the gut microbiome and atherosclerosisR01HL148577 · NHLBI · UNIVERSITY OF WISCONSIN-MADISON · PI LUSIS, ALDONS JAKE, REY, FEDERICO E · 2020 to 2023
$2.6M
Development of multifunctional probes for profiling microbial glycansU01CA231079 · NCI · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI IMPERIALI, BARBARA, KIESSLING, LAURA L · 2018 to 2020
$1.4M
NCI NIH HHS U01 CA231079NHLBI NIH HHS R01 HL148577NIAID NIH HHS R01 AI055258NIDDK NIH HHS P30 DK043351
6 · The paper itself

Abstract

Soluble human lectins are critical components of innate immunity. Genetic models suggest that lectins influence host-resident microbiota, but their specificity for commensal and mutualist species is understudied. Elucidating lectins' roles in regulating microbiota requires an understanding of which microbial species they bind within native communities. To profile human lectin recognition, we developed Lectin-Seq. We apply Lectin-Seq to human fecal microbiota using the soluble mannose-binding lectin (MBL) and intelectin-1 (hItln1). Although each lectin binds a substantial percentage of the samples (10 to 20%), the microbial interactomes of MBL and hItln1 differ markedly in composition and diversity. MBL binding is highly selective for a small subset of species commonly associated with humans. In contrast, hItln1's interaction profile encompasses a broad range of lower-abundance species. Our data uncover stark differences in the commensal recognition properties of human lectins.

Indexed as

Immunity, InnateLectinsHumansLectins

Identifiers

PMID37506208
PMCPMC10381928
OpenAlexW4385335152

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.