Evidence map›Paper›PMID 37505593›Full record

ArticleToxics2023

A Comparable icELISA and Lateral Flow Immunoassay for the Sensitive and Rapid Detection of 4,4'-Dinitrocarbanilide in Chicken.

Qianxin Liang, Chen Chen, Wenqing Xu, Ning Zhang, Jielin Yang, Wei Song, Huimei Cai, Ruyan Hou, Hongfang Li, Xiya Zhang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Toxics, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.0field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 1 country.

Qianxin LiangAnimal-Derived Food Safety Innovation Team, College of Tea and Food Science & Technology, Anhui Agricultural University, Hefei 230036, China.
Chen ChenAnimal-Derived Food Safety Innovation Team, College of Tea and Food Science & Technology, Anhui Agricultural University, Hefei 230036, China.
Wenqing XuAnimal-Derived Food Safety Innovation Team, College of Tea and Food Science & Technology, Anhui Agricultural University, Hefei 230036, China.
Ning ZhangAnimal-Derived Food Safety Innovation Team, College of Tea and Food Science & Technology, Anhui Agricultural University, Hefei 230036, China.
Jielin YangAnimal-Derived Food Safety Innovation Team, College of Tea and Food Science & Technology, Anhui Agricultural University, Hefei 230036, China.
Wei SongHefei Customs District Technical Center, Anhui Key Lab of Analysis and Detection for Food Safety, Hefei 230022, China.ORCID 0000-0001-8440-9492
Huimei CaiAnimal-Derived Food Safety Innovation Team, College of Tea and Food Science & Technology, Anhui Agricultural University, Hefei 230036, China.
Ruyan HouAnimal-Derived Food Safety Innovation Team, College of Tea and Food Science & Technology, Anhui Agricultural University, Hefei 230036, China.ORCID 0000-0003-4423-694X
Hongfang LiAnimal-Derived Food Safety Innovation Team, College of Tea and Food Science & Technology, Anhui Agricultural University, Hefei 230036, China.ORCID 0000-0002-9990-8259
Xiya ZhangCollege of Food Science and Technology, Henan Agricultural University, Zhengzhou 450002, China.
Anhui Agricultural University · CNAnhui Special Equipment Inspection Institute · CNHenan Agricultural University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

4,4'-dinitrocarbanilide (DNC) is a key component and marker residue of nicarbazin, which forms residues in edible tissue and then causes nephrotoxicity and hepatotoxicity in humans if used excessively. To simplify sample preparation and monitor the DNC rapidly and accurately, a comparable icELISA and lateral flow immunoassay (LFIA) was developed in this study. Briefly, the reaction parameters were explored for improving the sensitivity of icELISA and LFIA. Under the optimal conditions, methanol was selected as the extracting solvent for DNC in chicken, and 20- and 10-fold dilutions of sample extraction eliminated the matrix effect for icELISA and LFIA, separately. After sample pretreatment, the analysis properties of icELISA and LFIA were compared. The limit of detection of icELISA for DNC was 0.8 μg/kg, and the visual and quantitative limits of detection of LFIA were 8 and 2.5 μg/kg. Compared with icELISA, LFIA showed lower sensitivity but obvious advantages in terms of matrix tolerance and detection time (within 15 min). The sensitivity, specificity, and accuracy of the developed assays satisfied the detection requirement even if using simple sample pretreatment. This comparable icELISA and LFIA provided mutual verifiability methods for the accurate detection of DNC in chicken.

Indexed as

4,4′-dinitrocarbanilidechickenicELISAlateral flow immunoassayrapid detection

Identifiers

PMID37505593
PMCPMC10385411
OpenAlexW4384929759

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.