Evidence map›Paper›PMID 37504711›Full record

ArticleJournal of fungi (Basel, Switzerland)2023

Development of an Imaging Flow Cytometry Method for Fungal Cytological Profiling and Its Potential Application in Antifungal Drug Development.

Courtney L McMahon, Marisol Esqueda, Jieh-Juen Yu, Gina Wall, Jesus A Romo, Taissa Vila, Ashok Chaturvedi, Jose L Lopez-Ribot, Floyd Wormley, Chiung-Yu Hung

Open access · goldAbstract read
In one paragraph

Article in Journal of fungi (Basel, Switzerland), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
1.9field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 9 citations in OpenAlex.

  1. Drug Discovery and Repurposing forJournal of fungi (Basel, Switzerland) · 2025
    Review
  2. Article
  3. Article
  4. Review
  5. Journal of fungi (Basel, Switzerland) · 2024
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Courtney L McMahonDepartment of Molecular Microbiology and Immunology and South Texas Center for Emerging Infectious Diseases, The University of Texas at San Antonio, San Antonio, TX 78249, USA.ORCID 0000-0002-3523-6529
Marisol EsquedaDepartment of Molecular Microbiology and Immunology and South Texas Center for Emerging Infectious Diseases, The University of Texas at San Antonio, San Antonio, TX 78249, USA.
Jieh-Juen YuDepartment of Molecular Microbiology and Immunology and South Texas Center for Emerging Infectious Diseases, The University of Texas at San Antonio, San Antonio, TX 78249, USA.ORCID 0000-0001-8901-3366
Gina WallDepartment of Molecular Microbiology and Immunology and South Texas Center for Emerging Infectious Diseases, The University of Texas at San Antonio, San Antonio, TX 78249, USA.
Jesus A RomoDepartment of Molecular Microbiology and Immunology and South Texas Center for Emerging Infectious Diseases, The University of Texas at San Antonio, San Antonio, TX 78249, USA.ORCID 0000-0002-1683-2288
Taissa VilaDepartment of Molecular Microbiology and Immunology and South Texas Center for Emerging Infectious Diseases, The University of Texas at San Antonio, San Antonio, TX 78249, USA.
Ashok ChaturvediDepartment of Molecular Microbiology and Immunology and South Texas Center for Emerging Infectious Diseases, The University of Texas at San Antonio, San Antonio, TX 78249, USA.
Jose L Lopez-RibotDepartment of Molecular Microbiology and Immunology and South Texas Center for Emerging Infectious Diseases, The University of Texas at San Antonio, San Antonio, TX 78249, USA.ORCID 0000-0003-1992-3270
Floyd WormleyDepartment of Molecular Microbiology and Immunology and South Texas Center for Emerging Infectious Diseases, The University of Texas at San Antonio, San Antonio, TX 78249, USA.
Chiung-Yu HungDepartment of Molecular Microbiology and Immunology and South Texas Center for Emerging Infectious Diseases, The University of Texas at San Antonio, San Antonio, TX 78249, USA.ORCID 0000-0003-1091-3420
Texas Center for Infectious Disease · US

Funding

UTSA RISE Research Training ProgramR25GM060655 · NIGMS · UNIVERSITY OF TEXAS SAN ANTONIO · PI BAREA-RODRIGUEZ, EDWIN J · 2000 to 2022
$21.6M
Screening Repurposing Libraries for the Identification of Drugs with Novel anti-Coccidioidal ActivityU19AI166761 · NIAID · UNIVERSITY OF TEXAS SAN ANTONIO · PI CHIUNG-YU HUNG · 2022 to 2026
$8.1M
Development of a multivalent vaccine against Coccidioides infectionR01AI135005 · NIAID · UNIVERSITY OF TEXAS SAN ANTONIO · PI HUNG, CHIUNG-YU · 2018 to 2022
$1.9M
Screening a Target-Based Repurposing Library for Activity against Fungal Pathogens and Subsequent Preclinical Development of Leading CandidatesR33AI140823 · NIAID · UNIVERSITY OF TEXAS SAN ANTONIO · PI LOPEZ-RIBOT, JOSE L., WORMLEY, FLOYD L. · 2021 to 2023
$1.3M
Apply an imaging flow cytometry method to screen chemical libraries for identifying and repurposing novel drugs against coccidioidomycosisR21AI149015 · NIAID · UNIVERSITY OF TEXAS SAN ANTONIO · PI HUNG, CHIUNG-YU · 2020 to 2021
$403k
NIAID NIH HHS R01 AI135005NIAID NIH HHS R21 AI149015NIAID NIH HHS R33 AI140823NIAID NIH HHS U19 AI166761NIGMS NIH HHS R25 GM060655NIH HHS R21AI149015; R01AI135005; U19AI166761; R33AI140823
6 · The paper itself

Abstract

Automated imaging techniques have been in increasing demand for the more advanced analysis and efficient characterization of cellular phenotypes. The success of the image-based profiling method hinges on assays that can rapidly and simultaneously capture a wide range of phenotypic features. We have developed an automated image acquisition method for fungal cytological profiling (FCP) using an imaging flow cytometer that can objectively measure over 250 features of a single fungal cell. Fungal cells were labeled with calcofluor white and FM4-64FX, which bind to the cell wall and lipophilic membrane, respectively. Images of single cells were analyzed using IDEAS

Indexed as

antifungalcytological profilingdrug discoveryimaging flow cytometryphenotypic screening

Identifiers

PMID37504711
PMCPMC10381375
OpenAlexW4382940804

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.