Evidence map›Paper›PMID 37504121›Full record

ArticleBiosensors2023

Streptavidin-Conjugated DNA for the Boronate Affinity-Based Detection of Poly(ADP-Ribose) Polymerase-1 with Improved Sensitivity.

Fengli Gao, Gang Liu, Yishu Qiao, Xiuwen Dong, Lin Liu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biosensors, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.8field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 5 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Fengli GaoCollege of Chemistry and Chemical Engineering, Anyang Normal University, Anyang 455000, China.
Gang LiuCollege of Chemistry and Chemical Engineering, Anyang Normal University, Anyang 455000, China.
Yishu QiaoCollege of Chemistry and Chemical Engineering, Anyang Normal University, Anyang 455000, China.
Xiuwen DongCollege of Chemistry and Chemical Engineering, Anyang Normal University, Anyang 455000, China.
Lin LiuCollege of Chemistry and Chemical Engineering, Anyang Normal University, Anyang 455000, China.ORCID 0000-0001-7982-6005
Anyang Normal University · CNHenan University of Technology · CN

Funding

Program for Innovative Research Team of Science and Technology in the University of Henan Province 21IRTSTHN005
6 · The paper itself

Abstract

This work reports the development of a fluorescence method for the detection of poly(ADP-ribose) polymerase-1 (PARP1), in which a phenylboronic acid-modified fluorescein isothiocyanate dye (FITC-PBA) was used to recognize the formed poly(ADP-ribose) (PAR) polymer. The detection system was designed by conjugating recombinant streptavidin (rSA) with PARP1-specific double-stranded DNA (dsDNA) through streptavidin-biotin interaction. Capture of PARP1 via rSA-biotin-dsDNA allowed for the poly-ADP-ribosylation (PARylation) of both rSA and PARP1 in a homogeneous solution. The resulting rSA-biotin-dsDNA/PAR conjugates were then captured and separated via the commercialized nitrilotriacetic acid-nickel ion-modified magnetic bead (MB-NTA-Ni) through the interaction between NTA-Ni on MB surface and oligohistidine (His

Indexed as

BiotinPoly(ADP-ribose) PolymerasesDNAFluorescein-5-isothiocyanateStreptavidinBiotinDNAFluorescein-5-isothiocyanatePoly(ADP-ribose) PolymerasesStreptavidinboronic acidimmobilization-freemagnetic beadnitrilotriacetic acidpoly(ADP-ribose) polymerasestreptavidin

Identifiers

PMID37504121
PMCPMC10377026
OpenAlexW4383878572

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.